Abstract
Reactions of H(2)O(2) with superoxide dismutase were studied by e.p.r. (electron paramagnetic resonance) spectroscopy and other methods. In agreement with earlier work, the Cu(2+) of the enzyme is reduced by H(2)O(2), although the reaction does not go to completion and its kinetics are not simple. With dilute enzyme the time for half-reduction with 9mm-H(2)O(2) is about 150ms. It is suggested that the reaction is a one-electron reduction, involving liberation of O(2) (-). On somewhat more prolonged exposure to H(2)O(2), the enzyme is inactivated. For enzyme in dilute solution and over a limited range of H(2)O(2) concentrations, inactivation is first-order with respect to enzyme and reagent, with k=3.1m(-1).s(-1) at 20-25 degrees C. Inactivation is accompanied by marked changes in the e.p.r. and visible spectra and appears to be associated with destruction of one histidine residue per subunit. It is suggested that this histidine is close to the metal in the native enzyme and essential for its enzymic activity.
MeSH Terms
Amino Acids/analysis
Animals
Binding Sites
Cattle
Electron Spin Resonance Spectroscopy
Hydrogen Peroxide/pharmacology
Kinetics
Oxidation-Reduction
Protein Binding
Protein Conformation
Superoxide Dismutase/antagonists & inhibitors,blood
Time Factors
Chemicals
Amino Acids
Hydrogen Peroxide
Superoxide Dismutase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Bray R C
Cockle S A
Fielden E M
Roberts P B
Rotilio G
Calabrese L
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15 references, click to expand
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