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PMID: 4368746 Published · ppublish English Journal Article

Glycerol metabolism in Bacillus subtilis: gene-enzyme relationships.

Journal of bacteriology ·Vol. 119 ·No. 2 ·1974-08-00 ·Pages 431-42

Lindgren V, Rutberg L

Abstract

Bacillus subtilis mutants unable to catabolize glycerol (Glp mutants) were isolated and mapped. The location of the mutations on the chromosome was determined by a density transfer technique and confirmed by PBS1 transduction and transformation. The different mutations were ordered relative to each other. Mutations rendering the cells glycerol auxotrophic were also mapped and found not to be linked to the Glp mutations.

MeSH Terms
Bacillus subtilis/enzymology,metabolism Carbon Radioisotopes Cell-Free System Chromosome Mapping Enzyme Induction Genes Genetic Linkage Glucose/metabolism Glycerol/metabolism Glycerolphosphate Dehydrogenase/metabolism Mutagens Mutation Nitrosoguanidines Phosphotransferases/metabolism Thymidine/metabolism Thymine/metabolism Transduction, Genetic Transformation, Genetic Tritium
Chemicals
Carbon Radioisotopes Mutagens Nitrosoguanidines Tritium Glycerolphosphate Dehydrogenase Phosphotransferases Glucose Glycerol Thymine Thymidine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lindgren V
Rutberg L
References (35)
35 references, click to expand
  1. Membrane synthesis in Bacillus subtilis. II. Integration of membrane proteins in the absence of lipid synthesis.
    J Mol Biol. 1970 Apr 28;49(2):433-9 PMID: 4988528
  2. Induction of citrate transport in Bacillus subtilis during the absence of phospholipid synthesis.
    J Bacteriol. 1971 May;106(2):514-8 PMID: 4324807
  3. REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS.
    J Bacteriol. 1961 May;81(5):741-6 PMID: 16561900
  4. Chromosomal location of genes regulating resistance to bacteriophage in Bacillus subtilis.
    J Bacteriol. 1969 Jun;98(3):1087-97 PMID: 4977981
  5. Genetic mapping in Bacillus subtilis.
    J Mol Biol. 1967 Jul 14;27(1):163-85 PMID: 4962145
  6. Spontaneous release of DNA in sequential genetic order by Bacillus subtilis.
    J Mol Biol. 1969 Oct 14;45(1):137-52 PMID: 4981003
  7. The chemical composition of the cytoplasmic membrane of Bacillus subtilis.
    Eur J Biochem. 1967 Nov;2(4):448-53 PMID: 4295208
  8. Anaerobic L- -glycerophosphate dehydrogenase of Escherichia coli: its genetic locus and its physiological role.
    J Bacteriol. 1971 Dec;108(3):1224-34 PMID: 4945192
  9. The effect of aerobic metabolism on the inducible glycerol dehydrogenase of Aerobacter aerogenes.
    J Biol Chem. 1960 Jun;235:1824-9 PMID: 14417008
  10. Abnormal septation and inhibition of sporulation by accumulation of L- -glycerophosphate in Bacillus subtilis mutants.
    J Bacteriol. 1973 Feb;113(2):1034-45 PMID: 4632310
  11. The activation of glycerol dehydrogenase from Aerobacter aerogenes by monovalent cations.
    J Biol Chem. 1960 Jun;235:1820-3 PMID: 14417009
  12. Genetic control of L-alpha-glycerophosphate system in Escherichia coli.
    J Mol Biol. 1968 Feb 14;31(3):371-87 PMID: 4866330
  13. [Study of metabolism of glycerol by two mutants of Bacillus subtilis].
    Can J Microbiol. 1972 Aug;18(8):1315-25 PMID: 4626435
  14. Metabolic pathways of glycerol dissimilation; a comparative study of two strains of Aerobacter aerogenes.
    J Bacteriol. 1953 Nov;66(5):611-9 PMID: 13108864
  15. Further studies on the teichoic acid from Bacillus subtilis walls.
    Biochem J. 1961 Aug;80:254-61 PMID: 13684312
  16. Genetic analysis of pleiotropic negative sporulation mutants in Bacillus subtilis.
    J Bacteriol. 1971 Mar;105(3):896-901 PMID: 4994039
  17. Induction of the lactose transport system in a lipid-synthesis-defective mutant of Escherichia coli.
    J Bacteriol. 1970 Aug;103(2):410-6 PMID: 4914567
  18. Three kinds of controls affecting the expression of the glp regulon in Escherichia coli.
    J Bacteriol. 1973 Sep;115(3):816-23 PMID: 4580569
  19. [Permeation of glycerol and sporulation of Bacillus subtilis].
    Can J Microbiol. 1972 Aug;18(8):1307-13 PMID: 4626434
  20. Phospholipid composition of Bacillus subtilis.
    J Bacteriol. 1969 Jul;99(1):298-303 PMID: 4979443
  21. Utilization of L-alpha-glycerophosphate by Escherichia coli without hydrolysis.
    Proc Natl Acad Sci U S A. 1962 Dec 15;48:2145-50 PMID: 13930693
  22. THE SYNTHESIS OF TEICHOIC ACIDS. I. POLYGLYCEROPHOSPHATE.
    J Biol Chem. 1964 Oct;239:3168-77 PMID: 14245357
  23. Membrane synthesis in Bacillus subtilis. I. Isolation and properties of strains bearing mutations in glycerol metabolism.
    J Mol Biol. 1970 Apr 28;49(2):415-32 PMID: 4988527
  24. Sequential replication of the Bacillus subtilis chromosome. IV. Genetic mapping by density transfer experiment.
    J Mol Biol. 1967 Jul 28;27(2):349-68 PMID: 4964542
  25. Carbohydrate metabolism and transport in Bacillus subtilis. A study of ctr mutations.
    Mol Gen Genet. 1973 Mar 19;121(4):355-68 PMID: 4632931
  26. THE CONTROL OF DISSIMILATION OF GLYCEROL AND L-ALPHA-GLYCEROPHOSPHATE IN ESCHERICHIA COLI.
    J Biol Chem. 1964 Sep;239:3106-8 PMID: 14217903
  27. Pathway for oxidative dissimilation of glycerol in Bacillur subtilis.
    J Bacteriol. 1968 Aug;96(2):565-6 PMID: 4299863
  28. Growth stasis by accumulated L-alpha-glycerophosphate in Escherichia coli.
    J Bacteriol. 1965 Nov;90(5):1325-9 PMID: 5321485
  29. ACTIVE TRANSPORT OF L-ALPHA-GLYCEROPHOSPHATE IN ESCHERICHIA COLI.
    J Biol Chem. 1964 Sep;239:3098-105 PMID: 14217902
  30. The genetics of alkaline phosphatase formation in Bacillus subtilis.
    Genetics. 1965 Nov;52(5):1093-100 PMID: 4956914
  31. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  32. Chromosomal location of the structural gene for glycerol kinase in Escherichia coli.
    J Bacteriol. 1966 May;91(5):1763-6 PMID: 5327904
  33. CAPTURE OF GLYCEROL BY CELLS OF ESCHERICHIA COLI.
    Biochim Biophys Acta. 1965 Mar 29;94:479-87 PMID: 14314355
  34. Product induction of glycerol kinase in Escherichia coli.
    J Mol Biol. 1965 Dec;14(2):515-21 PMID: 4956458
  35. Phosphonomycin, a new antibiotic produced by strains of streptomyces.
    Science. 1969 Oct 3;166(3901):122-3 PMID: 5809587
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.119.2.431-442.1974
Published
1974-08-00
Pages
431-42
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC245625
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.119.2.431-442.1974 PMC Full Text View journal details
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