A deletion mutation placing the galactokinase gene of Escherichia coli under control of the biotin promoter.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 7 ·1974-07-00 ·Pages 2698-702

Ketner G, Campbell A

Abstract

A deletion has been isolated which fuses galK and presumably galT to the promoter and operator of the bioA operon. The deletion has endpoints in bioA and galE, and leaves the operator-proximal end of bioA intact and the regulatory sites themselves functional. In bacterial strains which carry the deletion, the expression of galK is regulated by biotin, although due to the inefficiency of the bioA promoter even the fully derepressed level of galactokinase is low. Electron micrographic examination of the mutation shows that it is a simple deletion, uncomplicated by other chromosomal abnormalities.

MeSH Terms

Biotin/metabolism Chromosome Mapping Coliphages Diphosphates/pharmacology Escherichia coli/enzymology,metabolism Galactose/metabolism Genes Genotype Microscopy, Electron Mutation Nucleic Acid Hybridization Operon Phosphotransferases/biosynthesis Transcription, Genetic Transduction, Genetic

Chemicals

Diphosphates Biotin Phosphotransferases Galactose

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Ketner G

Campbell A

References (27)

27 references, click to expand

Synthesis of 7-oxo-8-aminopelargonic acid, a biotin vitamer, in cell-free extracts of Escherichia coli biotin auxotrophs.
J Bacteriol. 1968 Oct;96(4):1291-7 PMID: 4879561
New mutations in the S cistron of bacteriophage lambda affecting host cell lysis.
Virology. 1969 May;38(1):200-2 PMID: 4891223
Divergent orientation of transcription from the biotin locus of Escherichia coli.
J Mol Biol. 1971 Feb 28;56(1):53-62 PMID: 4929887
A deletion analysis of prophage lambda and adjacent genetic regions.
Proc Natl Acad Sci U S A. 1968 Nov;61(3):956-62 PMID: 5246560
Increased frequency of deletions in DNA polymerase mutants of Escherichia coli.
Nature. 1970 Nov 14;228(5272):633-5 PMID: 4920019
The galactose operon of E. coli K-12. II. A deletion analysis of operon structure and polarity.
Genetics. 1969 Jun;62(2):249-64 PMID: 4905872
Nonessential functions of bacteriophage lambda.
Virology. 1969 Feb;37(2):177-88 PMID: 4974326
Biotin-requiring mutants of Escherichia coli K-12.
J Bacteriol. 1967 Dec;94(6):2065-6 PMID: 4864413
Transduction and segregation in Escherichia coli K12.
Virology. 1957 Oct;4(2):366-84 PMID: 13496552
Tryptophan messenger ribonucleic acid elongation rates and steady-state levels of tryptophan operon enzymes under various growth conditions.
J Mol Biol. 1970 Aug;51(3):541-50 PMID: 4923859
A transcription-initiating mutation within a structural gene of the tryptophan operon.
J Mol Biol. 1969 May 14;41(3):317-28 PMID: 4896018
A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane.
J Mol Biol. 1965 Jul;12(3):829-42 PMID: 4955314
Sensitive mutants of bacteriophage lambda.
Virology. 1961 May;14:22-32 PMID: 13690195
Genetic and biochemical analysis of the isoenzymes concerned in the first reaction of aromatic biosynthesis in Escherichia coli.
J Bacteriol. 1967 Jan;93(1):237-44 PMID: 5335890
Isolation of plaque-forming, galactose-transducing strains of phage lambda.
Genetics. 1972 Jun;71(2):189-206 PMID: 4558323
Location of promoter and operator sites in the biotin gene cluster of Escherichia coli.
Proc Natl Acad Sci U S A. 1972 Aug;69(8):2219-23 PMID: 4559599
Integration-negative mutants of bacteriophage lambda.
J Mol Biol. 1968 Feb 14;31(3):487-505 PMID: 5637199
Orientation of transcription for the galactose operon as determined by hybridization of gal mRNA with the separated DNA strands of coliphages lambda-dg.
J Mol Biol. 1968 Jul 14;35(1):207-13 PMID: 4939778
0 degree mutations in the galactose operon in E. coli. I. Genetic characterization.
Mol Gen Genet. 1967;100(2):178-89 PMID: 4869989
An early intermediate in the biosynthesis of biotin: Incorporation studies with [1,7-C(2)]pimelic acid.
Biochem J. 1966 Dec;101(3):601-6 PMID: 16742432
Deletion mutants of bacteriophage lambda. I. Isolation and initial characterization.
J Mol Biol. 1971 Mar 14;56(2):369-84 PMID: 4323931
Dethiobiotin synthesis from 7,8-diaminolargonic acid in cell-free extracts of a biotin auxotroph of Escherichia coli K-12.
J Biol Chem. 1969 Oct 25;244(20):5503-9 PMID: 4900015
Galactokinse from Escherichia coli.
J Biol Chem. 1963 Mar;238:873-8 PMID: 13977183
Genetics of the left arm of the chromosome of bacteriophage lambda.
Genetics. 1968 Jul;59(3):311-25 PMID: 5705826
Genetic and biochemical analysis of the biotin loci of Escherichia coli K-12.
J Bacteriol. 1968 Aug;96(2):515-24 PMID: 4877129
6-phosphogluconolactonase mutants of Escherichia coli and a maltose blue gene.
J Bacteriol. 1969 Dec;100(3):1296-301 PMID: 4902810
Linkage map of Escherichia coli strain K-12.
Bacteriol Rev. 1972 Dec;36(4):504-24 PMID: 4568762

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.71.7.2698

Published

1974-07-00

Pages

2698-702

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC388535

Subset

External Links

PubMed source DOI: 10.1073/pnas.71.7.2698 PMC Full Text View journal details

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A deletion mutation placing the galactokinase gene of Escherichia coli under control of the biotin promoter.

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