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PMID: 4363251 Published · ppublish English Journal Article

Polyadenylation of vesicular stomatitis virus mRNA.

Journal of virology ·Vol. 13 ·No. 5 ·1974-05-00 ·Pages 1055-60

Ehrenfeld E

Abstract

Vesicular stomatitis virus (VSV) mRNA isolated from infected cell polysomes contains polyadenylic acid [poly(A)] sequences. Detergent-activated purified virions in vitro can transcribe complementary RNA, which has sedimentation properties similar to mRNA, and this RNA also contains poly(A) sequences. Digestion of virion RNA with U2 RNase under conditions where hydrolysis is specific for purine linkages leaves no sequences of polyuridylic acid corresponding in length to the poly(A) on the transcripts. Growth of infectious virus is not inhibited by 3-deoxyadenosine (cordycepin) under conditions in which it inhibits polyadenylation of cellular mRNA. The virus-specific mRNA produced in the presence of cordycepin has poly(A) sequences of the same size distribution as that synthesized in the absence of cordycepin.

MeSH Terms
Adenosine Basidiomycota/enzymology Centrifugation, Density Gradient Electrophoresis, Polyacrylamide Gel Pancreas/enzymology Polynucleotides RNA, Messenger RNA, Viral Ribonucleases Tritium Uridine Vesicular stomatitis Indiana virus
Chemicals
Polynucleotides RNA, Messenger RNA, Viral Tritium Ribonucleases Adenosine Uridine
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Ehrenfeld E
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24 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.13.5.1055-1060.1974
Published
1974-05-00
Pages
1055-60
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC355414
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.13.5.1055-1060.1974 PMC Full Text View journal details
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