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PMID: 4363229 Published · ppublish English Journal Article

Leukocytosis-promoting factor of Bordetella pertussis. 3. Its identity with protective antigen.

Infection and immunity ·Vol. 9 ·No. 5 ·1974-05-00 ·Pages 801-10

Sato Y, Arai H, Suzuki K

Abstract

Leukocytosis-promoting factor (LPF) was purified from the supernatant of a 5-day liquid culture of Bordetella pertussis strains Tohama and no. 134. The preparations appeared to be filamentous molecules (2 by 40 nm) and contained histamine-sensitizing and hemagglutinin activities in addition to leukocytosis-promoting activity. Active protection tests in mice demonstrated strong protective activities of 80 international protective units per mg of protein in LPF preparations of each strain toxoided by mild Formalin treatment. Passive protection tests in mice with anti-LPF sera indicated that anti-LPF is one of the protective antibodies.

MeSH Terms
Agglutination Tests Animals Antibodies, Bacterial/isolation & purification Antigens, Bacterial Bacterial Proteins/analysis Bacteriological Techniques Bordetella pertussis/growth & development,immunology Female Formaldehyde/pharmacology Hemagglutination Inhibition Tests Hydrogen-Ion Concentration Immune Sera Immunity, Active Immunity, Maternally-Acquired Immunodiffusion Leukocytosis/immunology Mice Mice, Inbred Strains Microscopy, Electron
Chemicals
Antibodies, Bacterial Antigens, Bacterial Bacterial Proteins Immune Sera Formaldehyde
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sato Y
Arai H
Suzuki K
References (19)
19 references, click to expand
  1. Lymphocytosis and histamine sensitization of mice by fractions from Bordetella pertussis.
    J Bacteriol. 1968 Nov;96(5):1484-7 PMID: 4302297
  2. Interconnexion between the protective antigen and the histamine-sensitizing factor of Bordetella pertussis.
    Nature. 1960 Oct 22;188:331-2 PMID: 13790658
  3. The occurrence and properties of leukocytosis and lymphocytosis-stimulating material in the supernatant fluids of Bordetella pertussis cultures.
    J Exp Med. 1969 Mar 1;129(3):523-50 PMID: 4304138
  4. Effects of the lymphocytosis-promoting factor of Bordetella pertussis on antibody production in mice.
    Jpn J Med Sci Biol. 1969 Feb;22(1):23-42 PMID: 5307011
  5. An agglutinin-production test in the study of pertussis vaccines.
    J Pathol Bacteriol. 1953 Oct;66(2):479-88 PMID: 13118453
  6. Protective antigen of haemophilus pertussis.
    Lancet. 1954 Jun 19;266(6825):1257-60 PMID: 13164367
  7. The hemagglutinin of Haemophilus pertussis. II. Observations on the structure of the hemagglutinating complex of culture supernatants.
    Aust J Exp Biol Med Sci. 1950 Sep;28(5):509-16 PMID: 14820695
  8. A new growth medium for virulent Bordetella pertussis.
    J Pathol Bacteriol. 1961 Oct;82:431-8 PMID: 13918531
  9. Isolation of protective antigen from Bordetella pertussis.
    Biochem Biophys Res Commun. 1967 Apr 20;27(2):195-201 PMID: 4291987
  10. The soluble protective antigen and the histamine-sensitizing factor of Bordetella pertussis.
    J Immunol. 1965 Oct;95(4):643-50 PMID: 4284676
  11. Antigens of Bordetella pertussis. I. Activities of cell walls and protoplasm.
    J Immunol. 1959 Nov;83:496-501 PMID: 14425265
  12. Leucocytosis-promoting factor of Bordetella pertussis. I. Purification and characterization.
    Infect Immun. 1972 Dec;6(6):899-904 PMID: 4344632
  13. Leukocytosis-promoting factor of Bordetella pertussis. II. Biological properties.
    Infect Immun. 1973 Jun;7(6):992-9 PMID: 4351972
  14. Histamine-sensitizing factors from microbial agents, with special reference to Bordetella pertussis.
    Bacteriol Rev. 1968 Jun;32(2):103-26 PMID: 4298544
  15. The protective activity of components of Bordetella pertussis cell walls.
    Immunology. 1963 May;6:246-54 PMID: 13979394
  16. A Table Determining L.D.50 or the Fifty Per Cent End-Point.
    Proc Natl Acad Sci U S A. 1943 Jul;29(7):207-12 PMID: 16578076
  17. Studies on the ultrastructure of Bordetella pertussis. I. Morphology, origin, and biological activity of structures present in the extracellular fluid of liquid cultures of Bordetella pertussis.
    J Exp Med. 1970 Jun 1;131(6):1342-57 PMID: 4315895
  18. Antigen-antibody reactions in gels. IV. Types of reactions in coordinated systems of diffusion.
    Acta Pathol Microbiol Scand. 1953;32(2):230-40 PMID: 13079779
  19. Adsorption of protective antigen of Hemophilus pertussis on human red cell stromata.
    Proc Soc Exp Biol Med. 1950 Dec;75(3):704-5 PMID: 14808372
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.9.5.801-810.1974
Published
1974-05-00
Pages
801-10
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC414888
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.9.5.801-810.1974 PMC Full Text View journal details
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