Inhibition by ricin of protein synthesis in vitro: 60 S ribosomal subunit as the target of the toxin.

The Biochemical journal ·Vol. 136 ·No. 3 ·1973-11-00 ·Pages 813-5

Sperti S, Montanaro L, Mattioli A, Stirpe F

Abstract

Poly(U)-directed polyphenylalanine synthesis by rat liver ribosomes is strongly inhibited by ricin. Experiments involving hybridization between subunits derived from normal and ricin-treated ribosomes demonstrate that the 60S subunit is the site of action of the toxin. The toxin inactivates the 60S subunit independently of the presence of the 40S subunit.

MeSH Terms

Animals Carbon Radioisotopes Guanosine Triphosphate In Vitro Techniques Liver/cytology,metabolism Peptide Elongation Factors Phenylalanine/metabolism Phosphoric Monoester Hydrolases Plant Lectins Plants, Toxic Poly U/metabolism Protein Biosynthesis Rats Ribosomes/drug effects,metabolism Ricin/pharmacology Ricinus Toxins, Biological/pharmacology

Chemicals

Carbon Radioisotopes Peptide Elongation Factors Plant Lectins Toxins, Biological Poly U Phenylalanine Guanosine Triphosphate Ricin Phosphoric Monoester Hydrolases

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Sperti S

Montanaro L

Mattioli A

Stirpe F

References (11)

11 references, click to expand

Interaction of polypeptide chain elongation factors with rat liver ribosomal subunits.
J Mol Biol. 1969 Dec 28;46(3):447-57 PMID: 5365958
The function of 80 S ribosomal subunits and effects of some antibiotics.
Cold Spring Harb Symp Quant Biol. 1969;34:369-75 PMID: 5266173
The peptidyl transferase activity of ribosomes.
Cold Spring Harb Symp Quant Biol. 1969;34:357-68 PMID: 4909509
Thiostrepton: a ribosomal inhibitor of translocation.
Biochem Biophys Res Commun. 1970 Aug 11;40(3):667-74 PMID: 4321659
Ribosomes, G-factor and siomycin.
Nat New Biol. 1971 Mar 24;230(12):109-12 PMID: 4927374
Ricin - a potent inhibitor of protein synthesis.
FEBS Lett. 1972 Feb 15;20(3):327-329 PMID: 11946449
Comparison of amino acid polymerization factors isolated from rat liver and rabbit reticulocytes.
Arch Biochem Biophys. 1968 May;125(2):548-57 PMID: 4297868
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Dissociation of mammalian polyribosomes into subunits by puromycin.
Proc Natl Acad Sci U S A. 1971 Feb;68(2):390-4 PMID: 5277091
Treatment of abrin and ricin with -mercaptoethanol opposite effects on their toxicity in mice and their ability to inhibit protein synthesis in a cell-free system.
FEBS Lett. 1972 Nov 15;28(1):48-50 PMID: 4646874
Inhibition by ricin of protein synthesis in vitro. Ribosomes as the target of the toxin.
Biochem J. 1973 Nov;136(3):677-83 PMID: 4780693

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1360813

Published

1973-11-00

Pages

813-5

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1166019

Subset

External Links

PubMed source DOI: 10.1042/bj1360813 PMC Full Text View journal details

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Inhibition by ricin of protein synthesis in vitro: 60 S ribosomal subunit as the target of the toxin.

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