In vitro polyoma DNA synthesis: studies on an early temperature-sensitive mutant.

Journal of virology ·Vol. 13 ·No. 1 ·1974-01-00 ·Pages 241-3

Francke B, Hunter T

Abstract

The polyoma ts-a function was investigated by using an in vitro DNA-synthesizing system. A comparison of systems derived from ts25 (a ts-a group mutant)-and ts1260 (a late group mutant)-infected cells showed that the activation energies for DNA chain elongation and the mechanisms of discontinous growth were identical for both mutants.

MeSH Terms

Animals Cell Line Centrifugation, Density Gradient Cytosine Nucleotides/metabolism DNA/biosynthesis DNA Replication DNA, Viral/biosynthesis Mice Mutation Phosphorus Radioisotopes Polyomavirus/growth & development,metabolism Temperature Thymidine/metabolism Time Factors Tritium

Chemicals

Cytosine Nucleotides DNA, Viral Phosphorus Radioisotopes Tritium DNA Thymidine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Francke B

Hunter T

References (9)

9 references, click to expand

Selective extraction of polyoma DNA from infected mouse cell cultures.
J Mol Biol. 1967 Jun 14;26(2):365-9 PMID: 4291934
Complementation and transformation by temperature-sensitive mutants of polyoma virus.
Virology. 1969 May;38(1):120-5 PMID: 4306521
Intermediate in SV40 DNA chain growth.
Nat New Biol. 1972 Aug 30;238(87):274-7 PMID: 4342529
Simian virus 40 deoxyribonucleic acid synthesis: the viral replicon.
J Virol. 1972 Oct;10(4):591-8 PMID: 4343541
CELL-TRANSFORMING ABILITY OF A TEMPERATURE-SENSITIVE MUTANT OF POLYOMA VIRUS.
Proc Natl Acad Sci U S A. 1965 Mar;53:486-91 PMID: 14338226
Replication of polyoma DNA in isolated nuclei. I. Characterization of the system from mouse fibroblast 3T6 cells.
J Mol Biol. 1972 Dec 30;72(3):523-37 PMID: 4349757
Polyoma gene function required for viral DNA synthesis.
Virology. 1973 Sep;55(1):127-35 PMID: 4353950
In vitro polyoma DNA synthesis: characterization of a system from infected 3T3 cells.
J Virol. 1974 Jan;13(1):125-39 PMID: 4359420
RNA-linked short DNA fragments during polyoma replication.
Proc Natl Acad Sci U S A. 1973 Feb;70(2):412-5 PMID: 4346889

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.13.1.241-243.1974

Published

1974-01-00

Pages

241-3

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC355283

Subset

External Links

PubMed source DOI: 10.1128/JVI.13.1.241-243.1974 PMC Full Text View journal details

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In vitro polyoma DNA synthesis: studies on an early temperature-sensitive mutant.

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