A filter assay for thymidylate synthetase using 5-fluoro-2'-deoxyuridylate as an active site titrant.

Animals Binding Sites Carcinoma, Hepatocellular Cell-Free System Cytoplasm Deoxyribonucleotides Drug Resistance, Microbial Evaluation Studies as Topic Filtration Fluorine Kinetics Lactobacillus casei/enzymology Liver Neoplasms Methods Methotrexate Methyltransferases/analysis Neoplasms, Experimental Rats Spectrophotometry Tetrahydrofolates Thymidylate Synthase/analysis Tritium Uracil Nucleotides

Deoxyribonucleotides Tetrahydrofolates Uracil Nucleotides Tritium Fluorine Methyltransferases Thymidylate Synthase Methotrexate