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PMID: 4356466 Published · ppublish English Comparative Study Journal Article

Large-scale purification of ribonucleic acid tumor viruses by use of continuous-flow density gradient centrifugation.

Applied microbiology ·Vol. 26 ·No. 3 ·1973-09-00 ·Pages 452-4

Grandgenett DP, Brackmann K, Green M

Abstract

This report describes a 200- to 300-fold increase in the quantity of ribonucleic acud tumor virus particles previously isolated at one time by zonal centrifugation.

MeSH Terms
Avian Leukosis Virus/isolation & purification Centrifugation, Density Gradient Centrifugation, Zonal Gammaretrovirus/isolation & purification Methods Oncogenic Viruses/isolation & purification RNA Viruses/isolation & purification
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Grandgenett D P
Brackmann K
Green M
References (6)
6 references, click to expand
  1. Purification of the Moloney and Rauscher murine leukemia viruses by use of zonal ultracentrifuge systems.
    Appl Microbiol. 1967 May;15(3):582-9 PMID: 6035050
  2. K-series centrifuges. I. Development of the K-II continuous-sample-flow-with-banding centrifuge system for vaccine purification.
    Anal Biochem. 1969 Dec;32(3):460-94 PMID: 5361400
  3. Mechanism of carcinogenesis by RNA tumor viruses. I. An RNA-dependent DNA polymerase in murine sarcoma viruses.
    Proc Natl Acad Sci U S A. 1970 Sep;67(1):385-93 PMID: 4318786
  4. Polypeptides of avian RNA tumor viruses. 1. Isolation and physical and chemical analysis.
    Virology. 1970 Dec;42(4):1097-112 PMID: 4099078
  5. Large-volume purification of tumor viruses by use of zonal centrifuges.
    Appl Microbiol. 1972 May;23(5):1010-4 PMID: 4113253
  6. A single subunit from avian myeloblastosis virus with both RNA-directed DNA polymerase and ribonuclease H activity.
    Proc Natl Acad Sci U S A. 1973 Jan;70(1):230-4 PMID: 4119223
Article Info
Journal
Applied microbiology
Abbr.
Appl Microbiol
ISSN
0003-6919
DOI
10.1128/am.26.3.452-454.1973
Published
1973-09-00
Pages
452-4
Language
English
Region
United States
NLM ID
7605802
PMCID
PMC379819
Subset
IM
External Links
PubMed source DOI: 10.1128/am.26.3.452-454.1973 PMC Full Text
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