In vitro production of Clostridium perfringens enterotoxin and its detection by reversed passive hemagglutination.
The reversed passive hemagglutination (RPHA) test yielded a positive reaction in 2 h with as little as 0.5 ng of purified Clostridium perfringens enterotoxin (CPE) per ml as well as with cultures of some C. perfringens grown in Duncan-Strong (DS) medium. This method is the most sensitive, the simplest, and the fastest among all reported. The time course of CPE production of Clostridium perfringens NCTC 8798 in DS was investigated by RPHA. CPE in culture was detectable at 4 h, increased gradually, reached a maximum at 12 to 14 h, and remained at a high level of 20 mug/ml through 48 h of incubation. CPE synthesized within cells is released easily by sonic disruption of young cultures and by aging the cultures 20 h or more. Heat shock of the cell inoculum was essential for CPE production by C. perfringens in DS.
16 references, click to expand
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Assay methods for Clostridium perfringens type A enterotoxin.Appl Microbiol. 1973 Jul;26(1):111-5 PMID: 4353692
-
Erythemal activity of the cellular enteropathogenic factor of Clostridium perfringens type A.Can J Microbiol. 1970 Aug;16(8):651-4 PMID: 4320760
-
Rapid, sensitive assay for staphylococcal enterotoxin and a comparison of serological methods.Appl Microbiol. 1968 Jul;16(7):1019-23 PMID: 4969665
-
Improved medium for sporulation of Clostridium perfringens.Appl Microbiol. 1968 Jan;16(1):82-9 PMID: 4295179
-
Clostridium perfringens Type A Food Poisoning II. Response of the Rabbit Ileum as an Indication of Enteropathogenicity of Strains of Clostridium perfringens in Human Beings.Infect Immun. 1971 Jan;3(1):171-8 PMID: 16557937
-
Bioassay for -toxin of Clostridium perfrigens using survival time in mice.Jpn J Med Sci Biol. 1972 Feb;25(1):15-23 PMID: 4338979
-
Serological studies of types A, B, and E botulinal toxins by passive hemagglutination and bentonite flocculation.J Bacteriol. 1966 Mar;91(3):967-74 PMID: 5326104
-
Quantitation of Clostridium perfringens type A enterotoxin by electroimmunodiffusion.Appl Microbiol. 1972 Nov;24(5):801-4 PMID: 4344960
-
Sporulation and enterotoxin production by mutants of Clostridium perfringens.J Bacteriol. 1972 Apr;110(1):378-91 PMID: 4336110
-
Time of enterotoxin formation and release during sporulation of Clostridium perfringens type A.J Bacteriol. 1973 Feb;113(2):932-6 PMID: 4347930
-
Purification and characteristics of the enterotoxin of Clostridium perfringens type A.Can J Microbiol. 1971 Nov;17(11):1425-33 PMID: 4333491
-
Transient increase in capillary permeability induced by Clostridium perfringens type A enterotoxin.Infect Immun. 1972 Jan;5(1):147-50 PMID: 4348076
-
Clostridium perfringens Type A Food Poisoning I. Response of the Rabbit Ileum as an Indication of Enteropathogenicity of Strains of Clostridium perfringens in Monkeys.Infect Immun. 1971 Jan;3(1):167-70 PMID: 16557936
-
Ileal loop fluid accumulation and production of diarrhea in rabbits by cell-free products of Clostridium perfringens.J Bacteriol. 1969 Oct;100(1):86-94 PMID: 4310083
-
The micro-slide gel double diffusion test for the detection and assay of staphylococcal enterotoxins.Health Lab Sci. 1969 Oct;6(4):185-98 PMID: 4981479
-
Protein measurement with the Folin phenol reagent.J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
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