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PMID: 4355934 Published · ppublish English Journal Article

Membrane proteins of uninfected and Rous sarcoma virus- transformed avian cells.

Journal of virology ·Vol. 12 ·No. 2 ·1973-08-00 ·Pages 320-7

Bussell RH, Robinson WS

Abstract

A method for preparing large membrane fragments and cell ghosts was developed for uninfected and Rous sarcoma virus-transformed chicken embryo fibroblasts in culture. Membrane proteins were analyzed by electrophoresis in acrylamide gels containing sodium dodecyl sulfate. A major amino-acid-containing component of uninfected cell membranes was greatly diminished in amount or absent in membranes of virus-transformed cells. This component, called MP-1, had an electrophoretic mobility in sodium dodecyl sulfate-containing gels similar to that of a protein of a mol wt of 1.42 x 10(5). MP-1 was not altered by changes in cell growth rate or in cells infected with the nontransforming virus RAV-1.

MeSH Terms
Amino Acids Animals Avian Sarcoma Viruses/growth & development Carbon Radioisotopes Cell Fractionation Cell Membrane/analysis Cell Transformation, Neoplastic Cells, Cultured Centrifugation, Density Gradient Chick Embryo Electrophoresis, Polyacrylamide Gel Fibroblasts Fucose Glycoproteins/analysis Molecular Weight Neoplasm Proteins/analysis Proteins/analysis Satellite Viruses/growth & development Sodium Dodecyl Sulfate Spectrophotometry Thymidine Tritium
Chemicals
Amino Acids Carbon Radioisotopes Glycoproteins Neoplasm Proteins Proteins Tritium Fucose Sodium Dodecyl Sulfate Thymidine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bussell R H
Robinson W S
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28 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.12.2.320-327.1973
Published
1973-08-00
Pages
320-7
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC356626
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.12.2.320-327.1973 PMC Full Text View journal details
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