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PMID: 4352645 Published · ppublish English Journal Article

Response of Dictyostelium plasma membranes to adenosine 3':5'-cyclic monophosphate.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 6 ·1973-06-00 ·Pages 1630-3

Gregg JH, Nesom MG

Abstract

Myxamoebae of the cellular slime mold Dicytostelium discoideum aggregate in response to a chemotactic substance identified as adenosine 3':5'-cyclic monophosphate. Upon aggregation cell division is suppressed, the cells become adhesive, and differentiation is initiated. Freeze-fracture studies of myxamoebae were conducted to determine the effect of cyclic AMP and calcium on plasma membrane ultrastructure. The inner surfaces of the plasma membranes exhibited particulate structures whose sizes (43-187 A) and frequency distribution were determined. Cyclic AMP and calcium induced within 2 hr the formation of particles having average diameters 1.7-times and 1.5-times greater, respectively, than those of the vegetative myxamoebae controls. These data suggest that cyclic AMP mobilizes the intracellular calcium which may be effective in changing plasma membrane structure.

MeSH Terms
Calcium/pharmacology Cell Differentiation Cell Membrane/drug effects Cyclic AMP/pharmacology Freeze Etching Microscopy, Electron Myxomycetes/cytology,drug effects Time Factors
Chemicals
Cyclic AMP Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gregg J H
Nesom M G
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21 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.70.6.1630
Published
1973-06-00
Pages
1630-3
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC433560
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.70.6.1630 PMC Full Text View journal details
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