Evidence for the absence of the G-T-psi-C sequence from two mammalian initiator transfer RNAs.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 4 ·1973-04-00 ·Pages 1046-9

Petrissant G

Abstract

Analyses were performed on the purified initiator tRNA from rabbit liver to test for the presence of Psip and Tp in this molecule. Neither of these nucleotides could be detected after hydrolysis by piperidine, NaOH, or T2 RNase. Similarly digestion with venom phosphodiesterase plus phosphatase failed to release any pseudourdine or ribothymidine. Identical results were obtained with the initiator tRNA from sheep mammary gland. The absence of these nucleosides was confirmed by pancreatic and T1 RNase digestion of the rabbit-liver initiator tRNA. The classical G-T-Psi-C sequence was not detected in this molecule. An A-U-C-G sequence has been identified; it may possibly replace the G-T-Psi-C sequence.

MeSH Terms

Animals Base Sequence Chromatography, DEAE-Cellulose Chromatography, Gel Chromatography, Thin Layer Cytosine Nucleotides/analysis Escherichia coli/analysis Guanine Nucleotides/analysis Hydrolysis Liver/analysis Mammary Glands, Animal/analysis Nucleosides/analysis Nucleotides/analysis Oligonucleotides/analysis Phosphoric Diester Hydrolases Phosphoric Monoester Hydrolases Piperidines RNA, Transfer/analysis,isolation & purification Rabbits Ribonucleases Sheep Thymine Nucleotides/analysis

Chemicals

Cytosine Nucleotides Guanine Nucleotides Nucleosides Nucleotides Oligonucleotides Piperidines Thymine Nucleotides RNA, Transfer Ribonucleases Phosphoric Monoester Hydrolases Phosphoric Diester Hydrolases

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Petrissant G

References (22)

22 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.70.4.1046

Published

1973-04-00

Pages

1046-9

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC433422

Subset

External Links

PubMed source DOI: 10.1073/pnas.70.4.1046 PMC Full Text View journal details

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Evidence for the absence of the G-T-psi-C sequence from two mammalian initiator transfer RNAs.

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