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PMID: 4352108 Published · ppublish English Journal Article

Effects of proteolytic enzymes on function and structure of frog neuromuscular junctions.

The Journal of physiology ·Vol. 230 ·No. 3 ·1973-05-00 ·Pages 673-88

Betz W, Sakmann B

Abstract

1. Frog cutaneous pectoris nerve-muscle preparations were incubated with collagenase and protease and examined with electrophysiological and electron microscopic techniques.2. The physiological properties and intracellular ultrastructural appearance of individual muscle and nerve cells were not affected by the enzyme treatment. However, neuromuscular transmission and the morphology of the nerve-muscle junction were altered.3. Collagenase produced an irreversible loss of activity of end-plate cholinesterase and a partial loss of stainable ;synaptic cleft material'.4. Protease produced these changes and, in addition, the entire basement membrane was digested, which led to ;synaptic disjunction' of nerve terminals and muscle end-plates.

MeSH Terms
Acetylcholinesterase/analysis Action Potentials Animals Anura Basement Membrane/drug effects Cholinesterases/analysis Electrophysiology In Vitro Techniques Microbial Collagenase/pharmacology Microscopy, Electron Nerve Endings/drug effects Neuromuscular Junction/cytology,drug effects,enzymology Peptide Hydrolases/pharmacology Rana temporaria Synapses/drug effects Synaptic Transmission Time Factors
Chemicals
Acetylcholinesterase Cholinesterases Peptide Hydrolases Microbial Collagenase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Betz W
Sakmann B
References (31)
31 references, click to expand
  1. Electron microscope observations on the carbohydrate-rich cell coat present at the surface of cells in the rat.
    J Cell Biol. 1967 Jan;32(1):27-53 PMID: 10976200
  2. Dissociation of the 'surface membrane complex' in atrophic muscle fibres.
    Nature. 1959 Nov 7;184(Suppl 19):1507-8 PMID: 13800901
  3. Organization of the vestibular projection to the spinal cord of the cat.
    J Neurophysiol. 1966 Jul;29(4):626-64 PMID: 5298017
  4. The laminar organization of dorsal horn and effects of descending impulses.
    J Physiol. 1967 Feb;188(3):403-23 PMID: 6032207
  5. Pyramidal tract effects on interneurons in the cat lumbar dorsal horn.
    J Neurophysiol. 1968 Jan;31(1):69-80 PMID: 5640741
  6. Fine structural and cytochemical analysis of the staining of synaptic junctions with phosphotungstic acid.
    J Ultrastruct Res. 1968 Mar;22(5):361-75 PMID: 4173151
  7. Studies on the nature of the cholinergic receptor.
    Eur J Pharmacol. 1968 Aug;4(1):40-6 PMID: 5680369
  8. Isolation and characterization of the collagen from glomerular basement membrane.
    Biochemistry. 1968 Sep;7(9):3103-12 PMID: 5684337
  9. Demonstration of an intercellular substance in mouse cerebral cortex.
    Z Zellforsch Mikrosk Anat. 1967;81(3):366-73 PMID: 4177369
  10. Post-synaptic excitation and inhibition from primary afferents in neurones of the spinocervical tract.
    J Physiol. 1968 Dec;199(3):569-92 PMID: 5710423
  11. Responses of spinocervical tract neurones to natural stimulation of identified cutaneous receptors.
    Exp Brain Res. 1969;7(3):231-49 PMID: 5795249
  12. [Solubilization of electric organ acetylcholinesterase from eels. Action of trypsin].
    Eur J Biochem. 1970 Jul;14(3):430-9 PMID: 5488757
  13. Quantitation of human red blood cell fixation by glutaraldehyde.
    J Cell Biol. 1971 Jan;48(1):91-100 PMID: 5545112
  14. Enzymatic detachment of endplate acetylcholinesterase from muscle.
    Nat New Biol. 1971 Jul 14;232(28):62-3 PMID: 4327744
  15. [Ultrastructure of the muscle-tendon junction of skeletal and heart muscle].
    J Ultrastruct Res. 1971 Jul;36(1):68-85 PMID: 5568361
  16. "Disjunction" of frog neuromuscular synapses by treatment with proteolytic enzymes.
    Nat New Biol. 1971 Jul 21;232(29):94-5 PMID: 4328253
  17. Effects of descending impulses on transmission through the spinocervical tract.
    J Physiol. 1971 Dec;219(1):103-25 PMID: 4333861
  18. The cytochemistry of synaptic densities. II. Proteinaceous components and mechanism of synaptic connectivity.
    J Ultrastruct Res. 1971 Jun;35(5):451-75 PMID: 4111038
  19. Patterns of fiber degeneration in the lateral wall of the suprasylvian gyrus (Clare-Bishop area) following lesions in the visual cortex in cats.
    Brain Res. 1972 Aug 11;43(1):264-7 PMID: 5050194
  20. A method for dissection and electrical study in vitro of mammalian central nervous tissue.
    Science. 1951 Sep 21;114(2960):300-2 PMID: 14876427
  21. Some features of the ultrastructure of reptilian skeletal muscle.
    J Biophys Biochem Cytol. 1956 Jul 25;2(4):369-80 PMID: 13357501
  22. Three ascending spinal pathways in the dorsal part of the lateral funiculus.
    Acta Physiol Scand. 1961 Jan;51:1-16 PMID: 13764293
  23. Satellite cell of skeletal muscle fibers.
    J Biophys Biochem Cytol. 1961 Feb;9:493-5 PMID: 13768451
  24. Presynaptic inhibition of the central actions of flexor reflex afferents.
    J Physiol. 1962 May;161:258-81 PMID: 13889055
  25. Autoradiographic studies of the origin of the basement lamella in Ambystoma.
    Dev Biol. 1963 Mar;6:152-68 PMID: 13953244
  26. LOCAL, SEGMENTAL AND SUPRASPINAL INTERACTION WITH A DORSOLATERAL SPINAL CUTANEOUS AFFERENT SYSTEM.
    Exp Neurol. 1964 Oct;10:357-74 PMID: 14211932
  27. THE LOCALIZATION OF CHOLINESTERASE ACTIVITY IN RAT CARDIAC MUSCLE BY ELECTRON MICROSCOPY.
    J Cell Biol. 1964 Nov;23:217-32 PMID: 14222810
  28. PROPAGATION OF ELECTRIC ACTIVITY IN MOTOR NERVE TERMINALS.
    Proc R Soc Lond B Biol Sci. 1965 Feb 16;161:453-82 PMID: 14278408
  29. A SIMPLIFIED LEAD CITRATE STAIN FOR USE IN ELECTRON MICROSCOPY.
    J Cell Biol. 1965 May;25:407-8 PMID: 14287192
  30. Functional organization of the dorsal spino-cerebellar tract in the cat. I. Recording of mass discharge in dissected Flechsig's fasciculus.
    Acta Physiol Scand. 1956 Mar 24;36(1-2):175-87 PMID: 13313217
  31. Cell junctions in amphibian skin.
    J Cell Biol. 1965 Jul;26(1):263-91 PMID: 5859021
Article Info
Journal
The Journal of physiology
Abbr.
J Physiol
ISSN
0022-3751
DOI
10.1113/jphysiol.1973.sp010211
Published
1973-05-00
Pages
673-88
Language
English
Region
England
NLM ID
0266262
PMCID
PMC1350622
Subset
IM
External Links
PubMed source DOI: 10.1113/jphysiol.1973.sp010211 PMC Full Text View journal details
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