Purification by oligo(dT)-cellulose of viral-specific RNA from sarcoma virus-transformed mammalian nonproducer cells.

Journal of virology ·Vol. 11 ·No. 4 ·1973-04-00 ·Pages 600-2

Scolnick EM, Benveniste R, Parks WP

Abstract

Viral-specific RNA has been purified by oligo(dT)-cellulose chromatography from sarcoma virus-transformed nonproducer cells. This RNA comprises approximately 3% of the purified RNA, as judged by RNA-DNA hybridization.

MeSH Terms

Animals Cell Transformation, Neoplastic Cells, Cultured Cellulose Chromatography DNA, Viral Gammaretrovirus/growth & development Kidney Methods Nucleic Acid Hybridization RNA, Neoplasm/analysis,isolation & purification RNA, Viral/analysis,isolation & purification Rats Tritium

Chemicals

DNA, Viral RNA, Neoplasm RNA, Viral Tritium Cellulose

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Scolnick E M

Benveniste R

Parks W P

References (5)

5 references, click to expand

Virus specific RNA in cells transformed by RNA tumour viruses.
Nat New Biol. 1971 Apr 21;230(16):229-32 PMID: 5280402
An adenylic acid-rich sequence in messenger RNA of HeLa cells and its possible relationship to reiterated sites in DNA.
Proc Natl Acad Sci U S A. 1971 Jun;68(6):1321-5 PMID: 5288381
Characterization of murine sarcoma virus (Kirsten) transformation of mouse and human cells.
J Gen Virol. 1971 Nov;13(2):245-52 PMID: 4333712
Viral RNA subunits in cells transformed by RNA tumor viruses.
Science. 1972 Jun 30;176(4042):1418-20 PMID: 4338254
Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12 PMID: 4504350

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.11.4.600-602.1973

Published

1973-04-00

Pages

600-2

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC355143

Subset

External Links

PubMed source DOI: 10.1128/JVI.11.4.600-602.1973 PMC Full Text View journal details

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Purification by oligo(dT)-cellulose of viral-specific RNA from sarcoma virus-transformed mammalian nonproducer cells.

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