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PMID: 4347430 Published · ppublish English Journal Article

In vivo conversion of the single-stranded DNA of the kilham rat virus to a double-stranded form.

Journal of virology ·Vol. 11 ·No. 2 ·1973-02-00 ·Pages 299-305

Salzman LA, White W

Abstract

Kilham rat virus (KRV) contains linear, single-stranded DNA in the virion. The fate of radioactive viral DNA was followed after infection of monolayer cells. Within 60 min after infection of cells, 28 to 42% of the parental viral DNA is converted to a new form. This new DNA form is believed to be double stranded and linear on the basis of its sedimentation in neutral and alkaline sucrose gradients, elution from hydroxyapatite columns, its buoyant density in equilibrium CsCl density gradients, and appearance in the electron microscope. The double-stranded linear KRV DNA may be analogous to the replicative form of certain bacteriophages, including phiX174, which contain single-stranded circular genomes.

MeSH Terms
Animals Carbon Isotopes Cell Line Centrifugation, Density Gradient Chromatography Coliphages DNA Viruses/growth & development,metabolism DNA, Single-Stranded/analysis,metabolism DNA, Viral/analysis,isolation & purification,metabolism Hydroxyapatites Microscopy, Electron Molecular Weight Rats Thymidine Time Factors Tritium Virus Replication Wilms Tumor
Chemicals
Carbon Isotopes DNA, Single-Stranded DNA, Viral Hydroxyapatites Tritium Thymidine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Salzman L A
White W
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24 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.11.2.299-305.1973
Published
1973-02-00
Pages
299-305
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC355095
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.11.2.299-305.1973 PMC Full Text View journal details
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