Mutagenic DNA polymerase in human leukemic cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 1 ·1973-01-00 ·Pages 245-9

Springgate CF, Loeb LA

Abstract

Evidence is presented that DNA polymerases from human leukemic cells are mutagenic. Nucleic acid-free extracts of acute lymphoblastic leukemic cells polymerized about 10-times more dCTP using poly(dA-dT).poly(dA-dT) as a template than did extracts from normal lymphocytes. Mutagenic DNA polymerases could perhaps play an important role in tumor progression.

MeSH Terms

Adenosine Triphosphate/metabolism Centrifugation, Density Gradient Cytosine Nucleotides DNA Nucleotidyltransferases/blood DNA Replication DNA, Neoplasm/analysis,biosynthesis,metabolism Guanosine Triphosphate/metabolism Humans Leukemia, Lymphoid/enzymology Lymphocytes/enzymology Mutation Phosphoric Diester Hydrolases/metabolism Phosphorus Isotopes Polynucleotides/metabolism Templates, Genetic Thymine Nucleotides/metabolism Tritium

Chemicals

Cytosine Nucleotides DNA, Neoplasm Phosphorus Isotopes Polynucleotides Thymine Nucleotides Tritium Guanosine Triphosphate Adenosine Triphosphate DNA Nucleotidyltransferases Phosphoric Diester Hydrolases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Springgate C F

Loeb L A

References (15)

15 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.70.1.245

Published

1973-01-00

Pages

245-9

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC433224

Subset

External Links

PubMed source DOI: 10.1073/pnas.70.1.245 PMC Full Text View journal details

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