The time-course of the effects of ethanol on the redox and phosphorylation states of rat liver.

The Biochemical journal ·Vol. 127 ·No. 2 ·1972-04-00 ·Pages 387-97

Veech RL, Guynn R, Veloso D

Abstract

1. The time-course of the effects of ethanol administration on the metabolite concentrations, redox states and phosphorylation state was studied in the freeze-clamped liver of starved rats. The response was found to vary with the time after ethanol administration. 2. Administration of ethanol caused an immediate decrease in the [NAD(+)]/[NADH] ratio of both cytoplasm and mitochondria, which persisted over the 30min studied. 3. The free cytoplasmic [NADP(+)]/[NADPH] ratio in liver decreases immediately after ethanol administration but returns nearly to control values after 15min. 4. The cytoplasmic [ATP]/[ADP][HPO(4) (2-)] ratio is elevated 15min after ethanol administration in the starved rat. 5. The rapid and large changes in most metabolite concentrations measured appeared to result from the maintenance of near-equilibrium in a wide interlinked network. 6. Differences between fed and starved rats 15min after ethanol administration were slight.

MeSH Terms

Adenosine Diphosphate/analysis Adenosine Triphosphate/analysis Animals Cytoplasm/analysis Ethanol/pharmacology Liver/drug effects,enzymology Male Mitochondria, Liver/analysis NAD/analysis Oxidation-Reduction Oxidative Phosphorylation/drug effects Rats Starvation/metabolism Time Factors

Chemicals

NAD Ethanol Adenosine Diphosphate Adenosine Triphosphate

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Veech R L

Guynn R

Veloso D

References (27)

27 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1270387

Published

1972-04-00

Pages

387-97

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1178599

Subset

External Links

PubMed source DOI: 10.1042/bj1270387 PMC Full Text View journal details

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The time-course of the effects of ethanol on the redox and phosphorylation states of rat liver.

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