Properties of somatic cell hybrids between mouse cells and simian virus 40-transformed rat cells.

Journal of virology ·Vol. 10 ·No. 1 ·1972-07-00 ·Pages 67-72

van der Noordaa J, van Haagen A, Walboomers JM, van Someren H

Abstract

Hybrids between mouse cells and simian virus 40 (SV40)-transformed rat cells were made, and their properties and chromosome constitution were investigated over many generations. Their hybrid nature was confirmed by enzyme studies. During a period of 1 year a loss of 10 to 20% of the total number of chromosomes was observed. The SV40 tumor antigen was present and remained present in the hybrids. The parental and hybrid cells were studied for agglutination with concanavalin A, for growth in soft agar, and for serum requirement. These growth and surface characteristics of the transformed cells appeared in the hybrids.

MeSH Terms

Agglutination Animals Antigens, Viral/analysis Cell Line/enzymology,immunology Cell Transformation, Neoplastic Chromosomes Clone Cells Concanavalin A Culture Media Electrophoresis Fluorescent Antibody Technique Hybrid Cells/cytology,enzymology,immunology Immune Sera Indophenol L-Lactate Dehydrogenase/analysis Mice Oxidoreductases/metabolism Phosphogluconate Dehydrogenase/analysis Rats Rats, Inbred Strains Simian virus 40/immunology

Chemicals

Antigens, Viral Culture Media Immune Sera Concanavalin A Indophenol Oxidoreductases L-Lactate Dehydrogenase Phosphogluconate Dehydrogenase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

van der Noordaa J

van Haagen A

Walboomers J M

van Someren H

References (12)

12 references, click to expand

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Arch Biochem Biophys. 1971 Aug;145(2):470-83 PMID: 4331030
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Proc Natl Acad Sci U S A. 1969 Aug;63(4):1418-25 PMID: 4311253
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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.10.1.67-72.1972

Published

1972-07-00

Pages

67-72

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC356426

Subset

External Links

PubMed source DOI: 10.1128/JVI.10.1.67-72.1972 PMC Full Text View journal details

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Properties of somatic cell hybrids between mouse cells and simian virus 40-transformed rat cells.

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