Glucagon-stimulated phosphorylation of mitochondrial and lysosomal membranes of rat liver in vivo.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 4 ·1972-04-00 ·Pages 800-4

Zahlten RN, Hochberg AA, Stratman FW, Lardy HA

Abstract

The pancreatic hormone, glucagon, stimulates the net uptake of inorganic (32)P in vivo into rat liver and its incorporation into proteins of microsomes, mitochondrial membranes, and lysosomes. Incorporation into cytosolic proteins was enhanced only slightly by glucagon. More than 95% of the protein-bound phosphate is present as phosphoserine. Both the radioactivity and the total amount of protein-bound phosphate are increased after injection of glucagon. Glucagon treatment enhanced (32)P incorporation into the alcohol-ether soluble lipids of mitochondria but did not alter the relative distribution of (32)P in various phospholipid species.

MeSH Terms

Amino Acids/analysis Animals Butyrates/pharmacology Chromatography, Paper Cyclic AMP/pharmacology Fasting Glucagon/pharmacology Lipids/analysis Liver/cytology Lysosomes/analysis,metabolism Male Membranes/drug effects,metabolism Mitochondria, Liver/analysis,metabolism Oxidative Phosphorylation/drug effects Phosphates/analysis Phosphorus Isotopes Protein Binding/drug effects Rats Time Factors

Chemicals

Amino Acids Butyrates Lipids Phosphates Phosphorus Isotopes Glucagon Cyclic AMP

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Zahlten R N

Hochberg A A

Stratman F W

Lardy H A

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25 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.69.4.800

Published

1972-04-00

Pages

800-4

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC426567

Subset

External Links

PubMed source DOI: 10.1073/pnas.69.4.800 PMC Full Text View journal details

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Glucagon-stimulated phosphorylation of mitochondrial and lysosomal membranes of rat liver in vivo.

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