Metabolism of nicotinamide adenine dinucleotide in human and bovine strainsof Mycobacterium tuberculosis.

Journal of bacteriology ·Vol. 110 ·No. 2 ·1972-05-00 ·Pages 600-3

Kasărov LB, Moat AG

Abstract

A marked difference was found to exist between the nicotinamide adenine dinucleotide (NAD) glycohydrolase activity of human strains of Mycobacterium tuberculosis as compared with bovine strains. Human strains had from 6- to 20-fold higher NAD glycohydrolase activity than bovine strains. This finding explains the accumulation of free nicotinic acid in the culture medium by human strains and not by bovine strains. The biosynthetic intermediates nicotinic acid mononucleotide and deamido-NAD were not degraded by either human or bovine strains of M. tuberculosis; hence these nucleotides do not represent a source of the nicotinic acid accumulated by the human strains.

MeSH Terms

Amidohydrolases/metabolism Animals Carbon Isotopes Cattle Cell-Free System Chromatography, Paper Humans Mycobacterium bovis/enzymology,metabolism Mycobacterium tuberculosis/enzymology,metabolism N-Glycosyl Hydrolases/metabolism NAD/metabolism Niacinamide/metabolism Nicotinic Acids/biosynthesis,metabolism Nucleotides/metabolism Pentosyltransferases/metabolism Species Specificity

Chemicals

Carbon Isotopes Nicotinic Acids Nucleotides NAD Niacinamide Pentosyltransferases N-Glycosyl Hydrolases Amidohydrolases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Kasărov L B

Moat A G

References (14)

14 references, click to expand

Nicotinamide-adenine dinucleotide glycohydrolase of Mycobacterium tuberculosis H37Rv.
Biochem J. 1964 May;91(2):277-82 PMID: 4284486
Biosynthesis of NAD and nicotinic acid by Clostridium butylicum.
Biochem Biophys Res Commun. 1966 Mar 8;22(5):565-71 PMID: 4287786
Paper chromatography of nicotinic acid derivatives.
Nature. 1951 Sep 15;168(4272):475-7 PMID: 14875128
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
New chemical method to differentiate human-type tubercle bacilli from other mycobacteria.
Science. 1956 Nov 16;124(3229):985 PMID: 13380414
The metabolism of nicotinic acid in Mycobacteria: a method for differentiating tubercle bacilli of human origin from other Mycobacteria.
Am Rev Tuberc. 1957 Apr;75(4):529-37 PMID: 13411410
Hydrolysis of amides by extracts from Mycobacteria.
Biochem J. 1957 Apr;65(4):716-20 PMID: 13426090
Differentiation of human tubercle bacilli from atypical acid-fast bacilli. I. Niacin production of human tubercle bacilli and atypical acid-fast bacilli.
Am Rev Tuberc. 1958 Apr;77(4):669-74 PMID: 13521262
Differentiation of human tubercle bacilli from atypical acid-fast bacilli. II. Clinical application.
Am Rev Tuberc. 1958 Apr;77(4):675-80 PMID: 13521263
[Type differentiation of tuberculosis bacteria with the aid of the nicotinamidase test].
Tuberkulosearzt. 1959 Jun;13(6):377-84 PMID: 13676136
Differentiation of bovine tubercle bacilli from other mycobacteria by the determination of nicotinamidase activity.
Am Rev Respir Dis. 1960 Apr;81:550-4 PMID: 14410868
Nicotin-amide-adenine nucleotides of Mycobacterium tuberculosis H37Rv.
Biochem J. 1963 May;87:444-8 PMID: 13949147
Crystalline alcohol dehydrogenase from baker's yeast.
J Biol Chem. 1950 May;184(1):313-9 PMID: 15443900
NICOTINAMIDE ADENINE DINUCLEOTIDE SYNTHESIS BY MYCOBACTERIUM TUBERCULOSIS VAR. BOVIS.
Proc Soc Exp Biol Med. 1965 Jul;119:807-12 PMID: 14329008

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.110.2.600-603.1972

Published

1972-05-00

Pages

600-3

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC247454

Subset

External Links

PubMed source DOI: 10.1128/jb.110.2.600-603.1972 PMC Full Text View journal details

Analysis Services

Metabolism of nicotinamide adenine dinucleotide in human and bovine strainsof Mycobacterium tuberculosis.

Contact

WeChat Official Account