Abstract
Cyclic AMP-phosphodiesterase is present in various mouse fibroblasts. Contact-inhibited 3T3 cells contain two forms of the enzyme, one with a K(m) of 2.5 muM and the second with a K(m) of 71 muM. As 3T3 cells grow to confluency and cAMP concentrations rise, the activity of the first enzyme increases, whereas that of the second is unchanged. A line of SV40-transformed 3T3 cells with low cAMP concentration also has low levels of the cAMP-phosphodiesterase with a K(m) of 2.5 muM. Treatment of 3T3 and SV40-transformed 3T3 cells with dibutyryl cAMP and theophylline increases cAMP-phosphodiesterase accumulation. This accumulation is blocked by cycloheximide and actinomycin D. The newly formed enzyme resembles the higher affinity enzyme present in unstimulated cells, since it has a K(m) of 1.2-2.0 muM, and is stimulated by snake venom. In L cells in which cAMP concentrations are elevated by treatment with prostaglandin E(1), cAMP phosphodiesterase also accumulates. We conclude that intracellular concentrations of cAMP regulate the synthesis of cAMP-phosphodiesterase, and that cAMP functions as an inducer of the enzyme.
MeSH Terms
Animals
Butyrates/pharmacology
Cell Line
Contact Inhibition
Cyclic AMP/pharmacology
Cycloheximide/pharmacology
Dactinomycin/pharmacology
Depression, Chemical
Enzyme Activation/drug effects
Enzyme Induction/drug effects
Fibroblasts/drug effects,enzymology
Kinetics
L Cells/enzymology
Mice
Phosphoric Monoester Hydrolases/analysis,metabolism
Prostaglandins/pharmacology
Simian virus 40/cytology,metabolism
Stimulation, Chemical
Theophylline/pharmacology
Tritium
Chemicals
Butyrates
Prostaglandins
Tritium
Dactinomycin
Cycloheximide
Theophylline
Cyclic AMP
Phosphoric Monoester Hydrolases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
D'Armiento M
Johnson G S
Pastan I
References (9)
9 references, click to expand
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