Lateral diffusion of phospholipids in a vesicle membrane.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 10 ·1971-10-00 ·Pages 2564-8

Kornberg RD, McConnell HM

Abstract

Nuclear-resonance spectra of phosphatidylcholine (PC) vesicles are broadened by spin-labeled PC. The broadening of the N-methyl proton line is proportional to spin-labeled PC concentration; the broadening by 1 molecule in 100 of spin-labeled PC is 9.7 Hz at 35 degrees C; the broadening is not due to collisions between vesicles, exchange of spin label between vesicles, or fusion of vesicles. These findings are proof of rapid diffusion in the plane of the PC bilayer (lateral diffusion). A further study of the broadening reveals the molecular frequency of the translation step for lateral diffusion to be much greater than 3 x 10(3) sec(-1) at 0 degrees C.

MeSH Terms

Diffusion Eggs/analysis Electron Spin Resonance Spectroscopy Magnetic Resonance Spectroscopy Mathematics Membranes/analysis Phosphatidylcholines/analysis

Chemicals

Phosphatidylcholines

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Kornberg R D

McConnell H M

References (9)

9 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.68.10.2564

Published

1971-10-00

Pages

2564-8

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC389469

Subset

External Links

PubMed source DOI: 10.1073/pnas.68.10.2564 PMC Full Text View journal details

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Lateral diffusion of phospholipids in a vesicle membrane.

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