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PMID: 4331254 Published · ppublish English Journal Article

A comparison of mitochondria from Torulopsis utilis grown in continuous culture with glycerol, iron, ammonium, magnesium or phosphate as the growth-limiting nutrient.

The Biochemical journal ·Vol. 124 ·No. 1 ·1971-08-00 ·Pages 123-34

Light PA, Garland PB

Abstract

1. Mitochondria prepared from Torulopsis utilis grown in a chemostat with iron-limited growth were found to lack energy conservation but not electron flow in that segment of the respiratory chain leading from intramitochondrial NADH to the cytochromes [i.e. the site 1 segment (Lehninger, 1964)]. 2. Site 1 energy conservation was present in mitochondria prepared from cells grown under conditions of limitation by glycerol, ammonium and magnesium. Phosphate-limited growth resulted in mitochondrial preparations without respiratory control. 3. Mitochondria from cells grown under conditions of iron limitation were insensitive to the respiratory inhibitor piericidin A, whereas sensitivity was present in mitochondria prepared from glycerol-, ammonium-, magnesium- or phosphate-limited cells. 4. These observations are considered to provide indirect evidence for a role of non-haem iron in the mechanism of energy conservation and also piericidin A sensitivity in T. utilis mitochondria. 5. A readily constructed and inexpensive pH-measuring and -controlling circuit is described for use with continuous-culture apparatus.

MeSH Terms
Ammonia/metabolism Culture Media Cytochromes/metabolism Electron Transport Glycerol/metabolism Hydrogen-Ion Concentration Iron/metabolism Magnesium/metabolism Methods Mitochondria/metabolism Mitosporic Fungi/metabolism NAD/metabolism Oxygen Phosphates/metabolism Pyridines/pharmacology Temperature
Chemicals
Culture Media Cytochromes Phosphates Pyridines NAD Ammonia Iron Magnesium Glycerol Oxygen
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Light P A
Garland P B
References (47)
47 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1240123
Published
1971-08-00
Pages
123-34
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1177121
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1240123 PMC Full Text View journal details
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