Proteins of polyhedal cytoplasmic deoxyvirus. II. Nucleotide phosphohydrolase activity associated with frog virus 3.

Journal of virology ·Vol. 7 ·No. 5 ·1971-05-00 ·Pages 619-24

Vilagines R, McAuslan BR

Abstract

A nucleotide phosphohydrolase is firmly associated with a purified polyhedral cytoplasmic deoxyvirus, frog virus 3. This adenosine triphosphatase is distinguishable from known mammalian cell adenosine triphosphatases and from adenosine triphosphatase of an unrelated cytoplasmic replicating virus grown in the same host cell. The enzyme activity has a high specificity for adenosine triphosphate; the product of the reaction is adenosine diphosphate. The presence of similar activities in reovirus and poxvirus indicates that adenosine triphosphatase might have a function in the replication of these viruses.

MeSH Terms

Adenosine Diphosphate/biosynthesis Adenosine Triphosphatases/antagonists & inhibitors,metabolism Adenosine Triphosphate/metabolism Animals Anti-Bacterial Agents/pharmacology Anura Azides/pharmacology Cell Line Centrifugation, Density Gradient Chromatography, Paper Cricetinae DNA Viruses/enzymology,isolation & purification,metabolism Detergents/pharmacology Diphosphates/pharmacology Hydrogen-Ion Concentration Kidney Mercaptoethanol/pharmacology Ouabain/pharmacology Phosphates/pharmacology Phosphorus Isotopes Poxviridae/enzymology Rabbits Species Specificity Sucrose Temperature Tritium Virus Cultivation

Chemicals

Anti-Bacterial Agents Azides Detergents Diphosphates Phosphates Phosphorus Isotopes Tritium Sucrose Ouabain Mercaptoethanol Adenosine Diphosphate Adenosine Triphosphate Adenosine Triphosphatases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Vilagines R

McAuslan B R

References (17)

17 references, click to expand

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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.7.5.619-624.1971

Published

1971-05-00

Pages

619-24

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC356171

Subset

External Links

PubMed source DOI: 10.1128/JVI.7.5.619-624.1971 PMC Full Text View journal details

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Proteins of polyhedal cytoplasmic deoxyvirus. II. Nucleotide phosphohydrolase activity associated with frog virus 3.

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