Epinephrine-induced vacuole formation in parotid gland cells and its independence of the secretory process.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 1 ·1971-01-00 ·Pages 121-3

Batzri S, Amsterdam A, Selinger Z, Ohad I, Schramm M

Abstract

Catecholamines cause rapid release of K(+) and formation of vacuoles in acinar gland cells. A high K(+) concentration in the medium bathing parotid gland slices prevents vacuole formation by epinephrine and facilitates the secretion of most of the exportable amylase. N(6)-monobutyryl 3':5'-cyclic AMP does not cause K(+) release and vacuole formation although it efficiently induces amylase secretion. It is suggested that the secretory process is independent of vacuole formation.

MeSH Terms

Amylases/metabolism Animals Cyclic AMP/pharmacology Cytoplasm/drug effects Epinephrine/pharmacology In Vitro Techniques Microscopy, Electron Microscopy, Phase-Contrast Parotid Gland/cytology,drug effects,metabolism Potassium/metabolism Rats Stimulation, Chemical

Chemicals

Cyclic AMP Amylases Potassium Epinephrine

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Batzri S

Amsterdam A

Selinger Z

Ohad I

Schramm M

References (11)

11 references, click to expand

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The mechanism of enzyme secretion by the cell. 4. Effects of inducers, substrates and inhibitors on amylase secretion by rat parotid slices.
Eur J Biochem. 1967 Mar;1(1):96-101 PMID: 6059351
Energy production in rat parotid gland. Relation tonzyme secretion and effects of caium.
Eur J Biochem. 1970 Mar 1;13(1):158-63 PMID: 4314708
The action of adrenaline on serum potassium.
J Physiol. 1936 Feb 8;86(2):219-28 PMID: 16994748
Amylase secretion in rat parotid slices by apparent activation of endogenous catecholamine.
Biochim Biophys Acta. 1968 Oct 15;165(3):546-9 PMID: 4391088
The experimental production of watery vacuolation of the liver.
J Physiol. 1946 Dec 6;105(3):268-97 PMID: 16991727
Adenyl cyclase of rat parotid gland. Activation by fluoride and norepinephrine.
J Biol Chem. 1970 Jun;245(12):3225-31 PMID: 4988337
Early effects of 3',5'-adenosine monophosphate on the fluxes of calcium end potassium in the perfused liver of normal and adrenalectomized rats.
Proc Natl Acad Sci U S A. 1968 Oct;61(2):504-8 PMID: 4300988
Dynamic changes in the ultrastructure of the acinar cell of the rat parotid gland during the secretory cycle.
J Cell Biol. 1969 Jun;41(3):753-73 PMID: 5768873
The experimental production of watery vacuolation in the acinar cells of the submandibular gland.
J Physiol. 1967 Jan;188(2):191-205 PMID: 6030511
THE FUNCTION OF 3'5' CYCLIC AMP IN ENZYME SECRETION.
Biochem Biophys Res Commun. 1965 Feb 3;18:452-4 PMID: 14300765

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.68.1.121

Published

1971-01-00

Pages

121-3

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC391175

Subset

External Links

PubMed source DOI: 10.1073/pnas.68.1.121 PMC Full Text View journal details

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Epinephrine-induced vacuole formation in parotid gland cells and its independence of the secretory process.

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