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PMID: 4321344 Published · ppublish English Journal Article

Cell density-dependent changes of glycolipid concentrations in fibroblasts, and loss of this response in virus-transformed cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 67 ·No. 4 ·1970-12-00 ·Pages 1741-7

Hakomori S

Abstract

The glycolipids of normal fibroblasts cells at different stages of growth and with differing degrees of susceptibility to contact inhibition have been analyzed, as well as the glycolipids of virally transformed cells. The concentrations (per mg protein) of certain glycolipids, including galactosylgalactosyl-glucosyl-, N-acetylneuraminosylgalactosylglucosyl-, and N-acetylneuraminosyl-(N-acetylneuraminosyl)galactosylglucosyl-c eramide, increase on cell-to-cell contact of susceptible cells. The concentrations of these glycolipids decrease when susceptibility to contact inhibition is lost as a result of extensive passage in vitro or transformation by simian virus 40 or polyoma virus, and in all the malignant cells examined, the concentrations of these glycolipids were independent of cell density.

MeSH Terms
Animals Cell Line Cell Transformation, Neoplastic Chromatography, Thin Layer Contact Inhibition Cricetinae Fibroblasts/metabolism Glucose/metabolism Glycolipids/analysis,biosynthesis,metabolism Kidney Polyomavirus/metabolism Serine/metabolism Simian virus 40/metabolism Tritium
Chemicals
Glycolipids Tritium Serine Glucose
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Hakomori S
References (19)
19 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.67.4.1741
Published
1970-12-00
Pages
1741-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC283421
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.67.4.1741 PMC Full Text View journal details
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