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PMID: 4318954 Published · ppublish English Journal Article

Electron microscopy of chick fibroblasts infected by defective rous sarcoma virus and its helper.

Journal of virology ·Vol. 1 ·No. 2 ·1967-04-00 ·Pages 400-14

Courington D, Vogt PK

Abstract

Nonproducing Rous sarcoma cells of the chicken and their virus-producing as well as uninfected counterparts were studied with an electron microscope. The structural peculiarities of transformed cells included cytoplasmic annulate lamellae, aggregates of membrane-bound, glycogen-like granules, and empty, virus-like shells. Of 69 individual lines of nonproducing Rous sarcoma cells, 64 contained small numbers of viral particles. These particles were morphologically indistinguishable from mature avian tumor virus but lacked demonstrable infectivity. In sessile normal and leukosis virus-infected fibroblasts, microtubules and fibrils occurred in parallel arrays at the periphery of the cytoplasm. This cortical organization was absent from rounded Rous sarcoma cells. The characteristics of microtubular arrangement seemed to reflect differences in the locomotory activity of normal and transformed cells.

MeSH Terms
Animals Avian Sarcoma Viruses/growth & development Cell Transformation, Neoplastic Chickens Culture Techniques Fibroblasts Microscopy, Electron Microscopy, Fluorescence
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Courington D
Vogt P K
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43 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.1.2.400-414.1967
Published
1967-04-00
Pages
400-14
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC375238
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.1.2.400-414.1967 PMC Full Text View journal details
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