Home LiteratureArticle Details
PMID: 4316248 Published · ppublish English Journal Article

Specific role of each human leukocyte type in viral infections. I. Monocyte as host cell for vesicular stomatitis virus replication in vitro.

Journal of virology ·Vol. 1 ·No. 6 ·1967-12-00 ·Pages 1139-49

Edelman R, Wheelock EF

Abstract

Each major leukocyte type of the peripheral blood of healthy donors was studied in vitro for its ability to support vesicular stomatitis virus (VSV) replication. Purified cultures of each white blood cell type were prepared by the selective adsorption and elution of cells from silicone-treated glass beads. It was found that monocytes and macrophages (derived from the rapid transformation of monocytes in vitro) were the principal host cells for VSV replication. Interferon added to mixed leukocyte cultures, prior to virus inoculation, reduced virus yields and prevented destruction of macrophages. Cultures of small lymphocytes, containing no detectable monocytes or macrophages, produced amounts of virus equivalent to 1% of that produced in leukocyte cultures which contained 7% monocytes. Small lymphocytes did not undergo demonstrable cytopathic alterations in virus-infected cultures. VSV neither replicated nor produced cytopathic effects in polymorphonuclear leukocytes.

MeSH Terms
Culture Techniques Cytopathogenic Effect, Viral Humans Interferons/pharmacology Lymphocytes Macrophages Methods Monocytes Vesicular stomatitis Indiana virus/drug effects,growth & development,pathogenicity Virus Diseases/blood Virus Replication
Chemicals
Interferons
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Edelman R
Wheelock E F
References (32)
32 references, click to expand
  1. The cellular evolution of inflammatory granulomata.
    J Pathol Bacteriol. 1966 Jul;92(1):163-7 PMID: 5956253
  2. Virus replication and high-titered interferon production in human leukocyte cultures inoculated with Newcastle disease virus.
    J Bacteriol. 1966 Nov;92(5):1415-21 PMID: 5924272
  3. THE PATHOGENESIS OF HERPES VIRUS ENCEPHALITIS. II. A CELLULAR BASIS FOR THE DEVELOPMENT OF RESISTANCE WITH AGE.
    J Exp Med. 1964 Sep 1;120:359-74 PMID: 14207057
  4. ASPECTS OF THE PATHOGENESIS OF VIRUS DISEASES.
    Bacteriol Rev. 1964 Mar;28:30-71 PMID: 14127970
  5. Replication of mumps virus in human leukocyte cultures.
    J Bacteriol. 1966 Jul;92(1):258-65 PMID: 5328751
  6. Immunofluorescence study of the carrier state and mechanism of vertical transmission in lymphocytic choriomeningitis virus infection in mice.
    J Pathol Bacteriol. 1966 Apr;91(2):395-402 PMID: 5331383
  7. The Formation of Macrophages, Epithelioid Cells and Giant Cells from Leucocytes in Incubated Blood.
    Am J Pathol. 1925 Jan;1(1):91-100.1 PMID: 19969635
  8. Lymphocytes of small mammals: spontaneous transformation in culture to blastoids.
    Science. 1965 Sep 17;149(3690):1385-7 PMID: 5318095
  9. Rubella infection of human leukocytes. Chromosomal and viral studies.
    Am J Dis Child. 1965 Oct;110(4):473-6 PMID: 5834211
  10. THE DIFFERENTIATION OF MONONUCLEAR PHAGOCYTES. MORPHOLOGY, CYTOCHEMISTRY, AND BIOCHEMISTRY.
    J Exp Med. 1965 Jan 1;121:153-70 PMID: 14253481
  11. FACTORS AFFECTING THE DYE EXCLUSION TEST FOR CELL VIABILITY.
    Exp Cell Res. 1964 Jun;35:9-13 PMID: 14190667
  12. INTRACYTOPLASMIC VIRUS CRYSTAL FORMATION AND OTHER ALLTERATIONS IN POLYMORPHONUCLEAR LEUKOCYTES IN MOUSE POLIOENCEPHALITIS (MM STRAIN).
    Am J Pathol. 1964 Jan;44:29-49 PMID: 14103200
  13. MULTIPLICATION OF POLIOVIRUS TYPE I IN PREPARATIONS OF HUMAN LEUKOCYTES AND ITS INHIBITION BY INTERFERON.
    J Immunol. 1964 Jun;92:889-95 PMID: 14210796
  14. THE RAPID DIAGNOSIS OF VIRUS INFECTIONS BY IMMUNOFLUORESCENT TECHNIQUES APPLIED TO BLOOD LEUCOCYTES.
    Lancet. 1964 Apr 25;1(7339):911-2 PMID: 14124086
  15. MOUSE MACROPHAGES AS HOST CELLS FOR THE MOUSE HEPATITIS VIRUS AND THE GENETIC BASIS OF THEIR SUSCEPTIBILITY.
    Proc Natl Acad Sci U S A. 1960 Aug;46(8):1065-75 PMID: 16590714
  16. The regulation of pinocytosis in mouse macrophages. II. Factors inducing vesicle formation.
    J Exp Med. 1967 Feb 1;125(2):213-32 PMID: 4225263
  17. Metabolic basis of phagocytic activity.
    Physiol Rev. 1962 Jan;42:143-68 PMID: 14454025
  18. The development of histiocytes and macrophages from lymphocytes.
    J Lab Clin Med. 1955 Apr;45(4):499-507 PMID: 14368011
  19. Multiplication of ECHO 9 virus in suspensions of human leucocytes.
    Proc Soc Exp Biol Med. 1961 Dec;108:742-4 PMID: 13867594
  20. Relationships between viruses and leucocytes.
    Prog Med Virol. 1966;8:62-130 PMID: 4959446
  21. THE FORMATION OF MACROPHAGES FROM LYMPHOCYTES IN VITRO.
    Exp Cell Res. 1965 Jun;38:476-82 PMID: 14330676
  22. Reactions of influenza viruses with guinea pig polymorphonuclear leucocytes. I. Virus-cell interactions.
    Virology. 1956 Oct;2(5):618-36 PMID: 13371723
  23. GROWTH OF VIRULENT AND ATTENUATED ECTROMELIA VIRUS IN CULTURED MACROPHAGES FROM NORMAL AND ECTROMELIAIMMUNE MICE.
    J Immunol. 1964 Jun;92:837-42 PMID: 14210788
  24. Pathogenesis of rashes in virus diseases.
    Bacteriol Rev. 1966 Dec;30(4):739-60 PMID: 5342519
  25. Primary cultures of macrophages from normal human peripheral blood.
    Lab Invest. 1962 Dec;11:1322-31 PMID: 13970925
  26. The pathogenesis of infectious myxomatosis; the mechanism of infection and the immunological response in the European rabbit (Oryctolagus cuniculus).
    Br J Exp Pathol. 1953 Aug;34(4):400-11 PMID: 13093911
  27. Interferon production by mouse leukocytes in vitro and in vivo.
    J Exp Med. 1963 Jan 1;117:149-60 PMID: 13948365
  28. Studies on fetuin, a glycoprotein of fetal serum. I. Isolation, chemical composition, and physiochemical properties.
    J Biol Chem. 1960 Oct;235(10):2860-9 PMID: 16479679
  29. Vesicular stomatitis virus replication in human leukocyte cultures: enhancement by phytohemagglutinin.
    Science. 1966 Nov 25;154(3752):1053-5 PMID: 4288312
  30. Studies of cell source of macrophages from human blood in slide chambers.
    Proc Soc Exp Biol Med. 1962 Jul;110:429-31 PMID: 14489629
  31. Macrophages as a cellular expression of inherited natural resistance.
    Proc Natl Acad Sci U S A. 1962 Feb;48:160-5 PMID: 13900302
  32. Studies of canine distemper infection by means of fluorescein-labeled antibody. I. The pathogenesis, pathology, and diagnosis of the disease in experimentally infected ferrets.
    Virology. 1957 Feb;3(1):115-31 PMID: 13409764
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.1.6.1139-1149.1967
Published
1967-12-00
Pages
1139-49
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC375402
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.1.6.1139-1149.1967 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2