Formation of hydrogen peroxide by group N streptococci and its effect on their growth and metabolism.

Applied microbiology ·Vol. 19 ·No. 4 ·1970-04-00 ·Pages 608-12

Anders RF, Hogg DM, Jago GR

Abstract

The formation of hydrogen peroxide by group N streptococci was found to occur through the action of a reduced nicotinamide adenine dinucleotide (NADH) oxidase which catalyzed the oxidation of NADH by molecular oxygen. The enzyme was activated by flavine adenine dinucleotide. Whereas some of the hydrogen peroxide formed was removed through the action of an NADH peroxidase, sufficient accumulated in media to inhibit the growth, respiration, and viability of these organisms. The amount of hydrogen peroxide which accumulated varied among strains, and this variation could be related to differences in the properties of the NADH oxidase present.

MeSH Terms

Catalase/metabolism Cell-Free System Culture Media Depression, Chemical Enzyme Activation Flavin-Adenine Dinucleotide/pharmacology Glucose/metabolism Hydrogen Peroxide/biosynthesis,pharmacology Manometry NAD/metabolism Oxidoreductases/metabolism Oxygen Consumption Peroxidases/metabolism Spectrophotometry Streptococcus/drug effects,enzymology,growth & development,metabolism

Chemicals

Culture Media NAD Flavin-Adenine Dinucleotide Hydrogen Peroxide Oxidoreductases Peroxidases Catalase Glucose

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Anders R F

Hogg D M

Jago G R

References (7)

7 references, click to expand

Studies on the Respiratory Mechanism of the Streptococci.
J Bacteriol. 1935 Apr;29(4):411-35 PMID: 16559801
Pyridine nucleotide oxidizing enzymes of Lactobacillus casei. II. Oxidase and peroxidase.
Arch Biochem Biophys. 1965 Sep;111(3):534-9 PMID: 4285876
The inhibition of streptococci by lactoperoxidase, thiocyanate and hydrogen peroxide. The effect of the inhibitory system on susceptible and resistant strains of group N streptococci.
Biochem J. 1966 Aug;100(2):373-81 PMID: 4290983
Anti-streptococcal activity of lactoperoxidase III.
Proc Soc Exp Biol Med. 1962 Dec;111:585-8 PMID: 13964468
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
The inhibition of streptococci by lactoperoxidase, thiocyanate and hydrogen peroxide. The oxidation of thiocyanate and the nature of the inhibitory compound.
Biochem J. 1966 Aug;100(2):382-8 PMID: 5338806
Biological response of lactic streptococci and lactobacilli to catalase.
Appl Microbiol. 1969 Jun;17(6):797-800 PMID: 16349846

Article Info

Journal

Applied microbiology

Abbr.

Appl Microbiol

ISSN

0003-6919

DOI

10.1128/am.19.4.608-612.1970

Published

1970-04-00

Pages

608-12

Language

English

Region

United States

NLM ID

7605802

PMCID

PMC376748

Subset

External Links

PubMed source DOI: 10.1128/am.19.4.608-612.1970 PMC Full Text

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Formation of hydrogen peroxide by group N streptococci and its effect on their growth and metabolism.

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