Keywords
Adenosine Cyclic Monophosphate--analysis
Animals
Laboratory
Biology
Clinical Research
Comparative Studies
Lipid Metabolic Effects
Lipids
Physiology
Research Methodology
Studies
MeSH Terms
Adenine
Adenine Nucleotides/analysis,metabolism
Adipose Tissue/cytology,metabolism
Adrenocorticotropic Hormone/pharmacology
Animals
Antifungal Agents/pharmacology
Carbon Isotopes
Cholesterol/pharmacology
Chromatography, Paper
Cyclic AMP/analysis,metabolism
Depression, Chemical
Drug Synergism
Epididymis
Fatty Acids, Nonesterified/metabolism
Fluorides/pharmacology
Insulin/pharmacology
Lipid Metabolism
Male
Methods
Natamycin/pharmacology
Norepinephrine/pharmacology
Phosphatidylcholines/pharmacology
Prostaglandins/pharmacology
Puromycin/pharmacology
Rats
Stimulation, Chemical
Theophylline/pharmacology
Chemicals
Adenine Nucleotides
Antifungal Agents
Carbon Isotopes
Fatty Acids, Nonesterified
Insulin
Phosphatidylcholines
Prostaglandins
Puromycin
Natamycin
Adrenocorticotropic Hormone
Cholesterol
Theophylline
Cyclic AMP
Adenine
Fluorides
Norepinephrine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kuo J F
De Renzo E C
Other Abstracts
eng
A method for assaying, directly, cyclic 3',5'-adenosine (AMP) in isolated adipose cells is described which consists of measuring formation and accumulation of cyclic AMP-8 which is radiolabeled by 14C. This assay helped confirm known effects of cyclic AMP and adipose tissue. It showed that 1) cyclic AMP formation, stimulated by catecholamines, is evident before lipolysis if theophylline is also present
2) without theophylline, catecholamine-induced lipolysis occurred without elevating cyclic AMP levels
3) in the absence of norepinephrine, theophylline also induced lipolysis without elevating cyclic AMP levels
and 4) the synergistic effect of theophylline and norepinephrine on lipolysis induction was less marked than the corresponding synergism of theophylline and norepinephrine on elevating cyclic AMP levels. The assay was also used to confirm the decrease in cyclic AMP levels in adipocytes induced by insulin and prostaglandin E1. The data confirmed an hypothesis that cyclic AMP exists in different intracellular compartments, only 1 of which, comprising a small fraction of total cellular cyclic AMP, is involved in activation of the hormone-sensitive lipase. Also found was that polyene antibiotics lowered cellular cyclic AMP levels and markedly increased the levels observed in the medium.
