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PMID: 42918 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Cellular site of gastric acid secretion.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 12 ·1979-12-00 ·Pages 6689-93

DiBona DR, Ito S, Berglindh T, Sachs G

Abstract

Isolated gastric glands of the rabbit were examined both with differential interference-contrast microscopy and with electron microscopy to describe the morphologic correlates of acid secretion. Stimulation of the glands with histamine resulted in the development of intracellular spaces within the parietal cells. A similar transformation was produced by addition of 1 mM aminopyrine, whether the weak base was added in the presence of normal-K+ (5.4 mM) or high-K+ (108 mM) solutions. The intracellular space was compatible with the expanded canaliculus described in stimulated parietal cells. Confirmation that the space produced by histamine is the site of acid secretion was gained by combining fluorescence and interference-contrast methods in the presence of the dye acridine orange, which displays a pH-dependent metachromasia in its emission spectrum. Human gastrin I resulted in an observable discharge of peptic granules.

MeSH Terms

Acridine Orange Aminopyrine/pharmacology Animals Chlorides/metabolism Gastric Juice/metabolism Gastric Mucosa/cytology,metabolism Histamine/pharmacology Hydrogen-Ion Concentration Rabbits Secretory Rate/drug effects Stimulation, Chemical

Chemicals

Chlorides Aminopyrine Histamine Acridine Orange

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

DiBona D R

Ito S

Berglindh T

Sachs G

References (11)

11 references, click to expand

Direct visualization of epithelial morphology in the living amphibian urinary bladder.
J Membr Biol. 1978;40 Spec No:45-70 PMID: 104042
A nonelectrogenic H+ pump in plasma membranes of hog stomach.
J Biol Chem. 1976 Dec 10;251(23):7690-8 PMID: 12175
A method for preparing isolated glands from the rabbit gastric mucosa.
Acta Physiol Scand. 1976 Feb;96(2):150-9 PMID: 1258668
Effect of potassium on ion transport and bioelectric potentials of frog gastric mucosa.
Am J Physiol. 1958 Nov;195(2):499-504 PMID: 13583204
Use of a lipophilic cation for determination of membrane potential in neuroblastoma-glioma hybrid cell suspensions.
Proc Natl Acad Sci U S A. 1979 Feb;76(2):650-4 PMID: 284390
Fine structure of the stimulated oxyntic cell.
Fed Proc. 1965 Nov-Dec;24(6):1360-7 PMID: 5853154
Pronase method for isolation of viable cells from Necturus gastric mucosa.
Gastroenterology. 1971 Aug;61(2):189-200 PMID: 4104963
Some aspects of the problem of gastric hydrochloric acid secretion.
Arch Intern Med. 1972 Feb;129(2):270-8 PMID: 4110178
The membrane structure studied with cationic dyes. 1. The binding of cationic dyes to submitochondrial particles and the question of the polarity of the ion-translocation mechanism.
Eur J Biochem. 1972 Jan 21;24(3):553-65 PMID: 5058599
The zeiss-Nomarski differential interference equipment for transmitted-light microscopy.
Z Wiss Mikrosk. 1969 Nov;69(4):193-221 PMID: 5361069
Quantitation of hydrogen ion and potential gradients in gastric plasma membrane vesicles.
Biochemistry. 1978 Aug 8;17(16):3345-53 PMID: 28748

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.76.12.6689

Published

1979-12-00

Pages

6689-93

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC411934

Subset

External Links

PubMed source DOI: 10.1073/pnas.76.12.6689 PMC Full Text View journal details

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