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PMID: 42914 Published · ppublish English Journal Article

Structural order of lipids and proteins in membranes: evaluation of fluorescence anisotropy data.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 12 ·1979-12-00 ·Pages 6361-5

Jähnig F

Abstract

The limiting long-time value of fluorescence anisotropy in membranes is correlated with the orientational order parameter, which characterizes the structural anisotropy of membranes. Existing experimental results for diphenylhexatriene in lipid bilayers are evaluated for the order parameter of lipid order. Steady-state measurements of fluorescence anisotropy can provide the order parameter in good approximation. Proteins in a fluid lipid phase increase the lipid order parameter so determined. Upon comparison with the order parameter from deuterium magnetic resonance, it is concluded that proteins increase the order of the surrounding lipids in off-normal directions. Order parameters of protein order obtained from the limiting value of protein fluorescence anisotropy are discussed with respect to the influence of lipid order on protein order.

MeSH Terms
Fluorescence Polarization Hydrogen-Ion Concentration Kinetics Membrane Fluidity Membrane Lipids/physiology Membrane Proteins/physiology Membranes/ultrastructure Models, Theoretical Motion Temperature
Chemicals
Membrane Lipids Membrane Proteins
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Jähnig F
References (31)
31 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.12.6361
Published
1979-12-00
Pages
6361-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC411864
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.12.6361 PMC Full Text View journal details
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