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PMID: 4291236 Published · ppublish English Journal Article

Use of bisulfite in the streptococcal anti-nicotinamide adenine dinucleotidase test.

Applied microbiology ·Vol. 14 ·No. 3 ·1966-05-00 ·Pages 391-3

Ayoub EM, Ferretti JJ

Abstract

A major drawback in the performance of the streptococcal anti-nicotinamide adenine dinucleotidase test on a large scale has been the hazard involved in the use of cyanide in assaying nicotinamide adenine dinucleotide. The use of bisulfite instead of cyanide in the performance of this test was investigated. The assays obtained with bisulfite parallel closely those obtained with cyanide, and justify the replacement of cyanide by the safer bisulfite reagent.

MeSH Terms
Cyanides Indicators and Reagents NAD/metabolism Nucleotidases/metabolism Streptococcus/enzymology,immunology Sulfites
Chemicals
Cyanides Indicators and Reagents Sulfites NAD Nucleotidases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ayoub E M
Ferretti J J
References (5)
5 references, click to expand
  1. The reaction of pyridine nucleotide with cyanide and its analytical use.
    J Biol Chem. 1951 Aug;191(2):447-59 PMID: 14861191
  2. Neutralizing antibodies to streptococcal diphosphopyridine nucleotidase in the serum of experimental animals and human beings.
    J Exp Med. 1958 Sep 1;108(3):299-309 PMID: 13575667
  3. Serum inhibition of streptococcal diphosphopyridine nucleotidase in uncomplicated streptococcal pharyngitis and in rheumatic fever.
    J Clin Invest. 1959 Nov;38:1942-9 PMID: 13799742
  4. A FACTOR OTHER THAN STREPTOCOCCAL NICOTINAMIDE ADENINE DINUCLEOTIDASE WHICH COMBINES WITH ANTIBODY TO THIS ENZYME: ITS PRODUCTION AND EFFECT ON ANTIBODY DETERMINATIONS.
    J Immunol. 1963 May;90:793-803 PMID: 14053298
  5. Chemical properties of 3-substituted pyridine analogues of diphosphopyridine nucleotide.
    J Biol Chem. 1959 May;234(5):1219-25 PMID: 13654351
Article Info
Journal
Applied microbiology
Abbr.
Appl Microbiol
ISSN
0003-6919
Published
1966-05-00
Pages
391-3
Language
English
Region
United States
NLM ID
7605802
PMCID
PMC546724
Subset
IM
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