A pyruvate-valine enzyme complex that is dependent upon the metabolic state of the mitochondria.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 11 ·1974-11-00 ·Pages 4352-5

Bergquist A, Eakin EA, Murali DK, Wagner RP

Abstract

Data are presented suggesting that intact Neurospora crassa mitochondria contain an enzyme complex incorporating five enzymes necessary for the biosynthesis of isoleucine and valine. The functional integrity and stability of the complex has been shown to be dependent on the metabolic state of the mitochondria. The complex has been solubilized by digitonin and has an approximate molecular weight of 400,000.

MeSH Terms

Carbon Radioisotopes Hydro-Lyases/metabolism Isoleucine/biosynthesis Isomerases/metabolism Mitochondria/enzymology Molecular Weight Multienzyme Complexes/metabolism Neurospora/enzymology Neurospora crassa/enzymology,ultrastructure Oxo-Acid-Lyases/metabolism Transaminases/metabolism Valine/biosynthesis

Chemicals

Carbon Radioisotopes Multienzyme Complexes Isoleucine Transaminases Oxo-Acid-Lyases Hydro-Lyases Isomerases Valine

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Bergquist A

Eakin E A

Murali D K

Wagner R P

References (10)

10 references, click to expand

The pyruvate dehydrogenase complex from the mitochondrial fraction of Neurospora crassa.
Arch Biochem Biophys. 1970 Jun;138(2):653-61 PMID: 4317491
Synthesis of valine and isoleucine in the presence of a particulate cell fraction of Neurospora.
Proc Natl Acad Sci U S A. 1963 Jun;49:892-7 PMID: 13998269
Acetohydroxy acid synthetase with a pH optimum of 7.5 from Neurospora crassa mitochondria: characterization and partial purification.
J Bacteriol. 1972 Oct;112(1):453-64 PMID: 4263405
In vitro mitochondrial complementation in Neurospora crassa.
Biochem Genet. 1971 Dec;5(6):549-61 PMID: 5116521
Growth, respiratory, and cytochrome characteristics of certain of the isoleucine-valine mutants of Neurospora crassa.
Biochem Genet. 1974 Jul;12(1):39-49 PMID: 4279658
Purification and properties of dihydroxy acid dehydratase from soluble and mitochondrial fractions of Neurospora crassa.
Arch Biochem Biophys. 1970 May;138(1):160-70 PMID: 4986563
Molecular weight determination by chromatography on sepharose 4B.
FEBS Lett. 1969 Feb;2(4):242-244 PMID: 11946323
Branded chain amino acid aminotransferases of Neurospora crassa.
Arch Biochem Biophys. 1973 Mar;155(1):184-93 PMID: 4268214
Amino acid synthesis by the mitochondria of Neurospora crassa. I. Dependence on respiration of mitochondria.
Arch Biochem Biophys. 1969 Nov;134(2):401-7 PMID: 5354770
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.71.11.4352

Published

1974-11-00

Pages

4352-5

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC433881

Subset

External Links

PubMed source DOI: 10.1073/pnas.71.11.4352 PMC Full Text View journal details

Analysis Services

A pyruvate-valine enzyme complex that is dependent upon the metabolic state of the mitochondria.

Contact

WeChat Official Account