Localization of five antibiotic resistances at the subunit level in chloroplast ribosomes of Chlamydomonas.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 5 ·1974-05-00 ·Pages 1715-9

Schlanger G, Sager R

Abstract

The chloroplast ribosomes from five antibiotic resistant strains of Chlamydomonas, each carrying one mutant gene mapping in chloroplast DNA, have been shown to be resistant to the corresponding antibiotic in a poly(U)-directed amino-acid incorporating assay system. The alteration conferring resistance was localized to the 30S subunit in ribosomes from streptomycin, neamine, and spectinomycin resistant strains, and to the 50S subunit in ribosomes from cleocin and carbomycin resistant strains. Spectinomycin resistant ribosomes showed no cross-resistance to any other drugs, but limited cross-resistance was noted with the other mutant ribosomes. The similarity between these findings and results reported by others with bacterial ribosomes supports our hypothesis that at least some chloroplast ribosomal proteins are coded by genes in chloroplast DNA.

MeSH Terms

Chlamydomonas Chloroplasts Clindamycin/pharmacology Cross Reactions Drug Resistance Genes In Vitro Techniques Leucomycins/pharmacology Neomycin/pharmacology Phenylalanine/metabolism Plant Proteins/biosynthesis Ribosomes/metabolism Spectinomycin/pharmacology Streptomycin/pharmacology

Chemicals

Leucomycins Plant Proteins Clindamycin Phenylalanine Spectinomycin Neomycin Streptomycin

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Schlanger G

Sager R

References (25)

25 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.71.5.1715

Published

1974-05-00

Pages

1715-9

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC388309

Subset

External Links

PubMed source DOI: 10.1073/pnas.71.5.1715 PMC Full Text View journal details

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Localization of five antibiotic resistances at the subunit level in chloroplast ribosomes of Chlamydomonas.

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