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PMID: 4275705 Published · ppublish English Journal Article

Experimental diabetic ketoacidosis. Sequential changes of metabolic intermediates in blood, liver, cerebrospinal fluid and brain after acute insulin deprivation in the streptozotocin-diabetic rat.

The Biochemical journal ·Vol. 138 ·No. 1 ·1974-01-00 ·Pages 107-17

Blackshear PJ, Alberti KG

Abstract

Male rats rendered diabetic by the intravenous injection of streptozotocin (150mg/kg) were treated with a long-acting insulin for 1 week, then allowed to develop ketoacidosis. By using sampling techniques designed to avoid the use of anaesthesia and extended anoxic periods, sequential measurements of metabolic intermediates were made in blood, liver, cerebrospinal fluid and brain at 24h intervals after the last insulin injection. Measurements in blood and liver suggested a rapid increase in hepatic glycogenolysis and gluconeogenesis and peripheral-depot lipolysis between 24 and 48h after the last insulin injection, whereas blood and liver ketone-body and triglyceride concentrations rose more slowly. The changing metabolic patterns occurring with increasing time of insulin deprivation stress the importance of sequential compared with static measurements in experimental diabetes. Data are presented for brain metabolic intermediates in diabetic ketoacidosis, and support recent evidence that glucose plays a less important role in brain oxidative metabolism in ketotic states.

MeSH Terms
Animals Antibodies Blood Glucose Body Weight Brain/drug effects,metabolism Diabetes Mellitus/chemically induced Diabetic Ketoacidosis/blood,cerebrospinal fluid,metabolism Gluconeogenesis Glycogen/metabolism Insulin/pharmacology Ketone Bodies/biosynthesis Lipid Mobilization Liver/drug effects,metabolism Male Organ Size Radioimmunoassay Rats Streptozocin Time Factors Triglycerides/biosynthesis
Chemicals
Antibodies Blood Glucose Insulin Ketone Bodies Triglycerides Streptozocin Glycogen
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Blackshear P J
Alberti K G
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38 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1380107
Published
1974-01-00
Pages
107-17
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1166180
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1380107 PMC Full Text View journal details
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