Uptake of nitrite by Neurospora crassa.

Journal of bacteriology ·Vol. 118 ·No. 1 ·1974-04-00 ·Pages 270-4

Schloemer RH, Garrett RH

Abstract

Like the nitrate transport system, the nitrite uptake system in Neurospora crassa is induced by either nitrate or nitrite. This induction is prevented by cycloheximide, puromycin, or 6-methyl purine. The K(m) for nitrite of the induced nitrite uptake system is 86 muM, and the V(max) is 100 mumol of nitrite per g (wet weight) per h. Nitrite uptake is inhibited by metabolic poisons such as arsenate, dinitrophenol, cyanide, and antimycin A. No repression or inhibition of the nitrite transport system by ammonia, nitrate, or Casamino Acids was observed.

MeSH Terms

Amino Acids/metabolism Ammonia/metabolism Antimycin A/pharmacology Arsenic/pharmacology Biological Transport, Active Cell-Free System Culture Media Cyanides/pharmacology Cycloheximide/pharmacology Dactinomycin/pharmacology Dinitrophenols/pharmacology Enzyme Induction Enzyme Repression Neurospora/metabolism Neurospora crassa/enzymology,metabolism Nitrate Reductases/metabolism Nitrates/metabolism Nitrite Reductases/metabolism Nitrites/metabolism Purines/pharmacology Puromycin/pharmacology

Chemicals

Amino Acids Culture Media Cyanides Dinitrophenols Nitrates Nitrites Purines Dactinomycin Puromycin Antimycin A Ammonia Cycloheximide Nitrate Reductases Nitrite Reductases Arsenic

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Schloemer R H

Garrett R H

References (10)

10 references, click to expand

The effects of 2,4-dinitrophenol and other uncoupling agents on the assimilation of nitrate and nitrite by chlorella.
Biochim Biophys Acta. 1968 Jul 16;162(1):32-8 PMID: 5665259
Dinitrophenol as a catalyst of photosynthetic phosphorylation.
Biochim Biophys Acta. 1959 Nov;36:264-5 PMID: 13844109
Nitrate transport system in Neurospora crassa.
J Bacteriol. 1974 Apr;118(1):259-69 PMID: 4274457
The enzymic reduction of nitrite to ammonia by reduced pyridine nucleotides.
Biochim Biophys Acta. 1954 Sep;15(1):159-61 PMID: 13198962
Intact tissue assay for nitrite reductase in barley aleurone layers.
Plant Physiol. 1971 Jun;47(6):790-4 PMID: 16657706
Inhibition of CO2 fixation by nitrous acid.
Biochim Biophys Acta. 1965 Nov 29;109(2):607-10 PMID: 5867560
The induction of nitrite reductase in Neurospora crassa.
Biochim Biophys Acta. 1972 May 16;264(3):481-9 PMID: 4402141
Regulation of nitrate reductase in Neurospora crassa: regulation of transcription and translation.
J Bacteriol. 1972 May;110(2):547-53 PMID: 4259976
Reduction of nitrate and nitrite by subcellular preparations of Anabaena cylindrica. I. Reduction of nitrite to ammonia.
Plant Physiol. 1966 Jun;41(6):1031-6 PMID: 5945126
Ferredoxin:nitrite oxidoreductase from Chlorella. Purification and properties.
Biochim Biophys Acta. 1972 Aug 28;276(2):363-75 PMID: 5068817

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.118.1.270-274.1974

Published

1974-04-00

Pages

270-4

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC246666

Subset

External Links

PubMed source DOI: 10.1128/jb.118.1.270-274.1974 PMC Full Text View journal details

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Uptake of nitrite by Neurospora crassa.

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