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PMID: 4270437 Published · ppublish English Journal Article

Physiochemical characterization of a neonatal calf diarrhea virus.

Canadian journal of comparative medicine : Revue canadienne de medecine comparee ·Vol. 37 ·No. 3 ·1973-07-00 ·Pages 295-301

Welch AB, Thompson TL

Abstract

Physicochemical characteristics of two isolates of a neonatal calf diarrhea virus were investigated. Neither isolate was sensitive to ether or chloroform, both were stable at pH 3.0, were relatively heat resistant, but were thermolabile when heated to 50 degrees C for one hour in the presence of 1.0 M MgCl(2). Multiplication of virus was not inhibited by concentrations of 5-iodo-2'-deoxyuridine (IDUR) up to 500 microg/ml, which indicated that the nucleic acid was ribonucleic acid (RNA). Also, multiplication was not inhibited by concentrations of actinomycin D (AD) up to 0.5 microg/ml. Thermal denaturation studies demonstrated that the nucleic acid had a high melting temperature. Resistance to lipid solvents, stability at an acid pH, relatively high thermostability, type of nucleic acid, plus previous reports from this laboratory on general morphology and cytopathogenicity suggest that the virus may belong to the diplornavirus (reovirus) group. However, thermolability in the presence of 1.0 M MgCl(2) is not consistent with characteristics of this group.

MeSH Terms
Animals Animals, Newborn Cattle Cattle Diseases/microbiology Cells, Cultured Chlorides/pharmacology Chloroform/pharmacology Dactinomycin/pharmacology Diarrhea/microbiology,veterinary Embryo, Mammalian Erythrocytes/immunology Ethyl Ethers/pharmacology Hemagglutination Hydrogen-Ion Concentration Idoxuridine/pharmacology Kidney Magnesium/pharmacology RNA Viruses/drug effects,immunology,isolation & purification Reoviridae/drug effects,immunology Temperature Virus Diseases/microbiology,veterinary
Chemicals
Chlorides Ethyl Ethers Dactinomycin Chloroform Magnesium Idoxuridine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Welch A B
Thompson T L
References (21)
21 references, click to expand
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Article Info
Journal
Canadian journal of comparative medicine : Revue canadienne de medecine comparee
Abbr.
Can J Comp Med
ISSN
0008-4050
Published
1973-07-00
Pages
295-301
Language
English
Region
Canada
NLM ID
0151747
PMCID
PMC1319775
Subset
IM
External Links
PubMed source PMC Full Text
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