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PMID: 4270109 Published · ppublish English Journal Article

The alkaline cleavage and borohydride reduction of cartilage proteoglycan.

The Biochemical journal ·Vol. 133 ·No. 3 ·1973-07-00 ·Pages 457-70

Robinson HC, Hopwood JJ

Abstract

A method for the rapid isolation and purification of proteoglycan by using neutral solutions of LiBr for extraction and density-gradient centrifugation is described. The effect of 0.5m-KOH on isolated proteoglycan has been studied by using NaB(3)H(4) to reduce and label the chondroitin sulphate chains released. This study has established: (a) that at least 95% of the chondroitin sulphate chains are attached to the proteoglycan by alkali-labile bonds between xylose and serine; (b) that random degradation of the chondroitin sulphate chains does not occur to any significant extent; (c) that the method is convenient for the determination of polysaccharide number-average molecular weights.

MeSH Terms
Animals Borohydrides Cartilage Cattle Centrifugation, Density Gradient Chondroitin/isolation & purification Glycosaminoglycans/analysis Hydrolysis Hydroxides Molecular Weight Nasal Septum Oxidation-Reduction Serine Ultracentrifugation Xylose
Chemicals
Borohydrides Glycosaminoglycans Hydroxides Serine Chondroitin Xylose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Robinson H C
Hopwood J J
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23 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1330457
Published
1973-07-00
Pages
457-70
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1177724
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1330457 PMC Full Text View journal details
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