An evaluation of 12 methods for the demonstration of penicillinase.

Journal of clinical pathology ·Vol. 32 ·No. 10 ·1979-10-00 ·Pages 1061-5

Lucas TJ

Abstract

Twelve methods for the demonstration of bacterial penicillinase production by strains of Haemophilus influenzae and Staphylococcus aureus are compared, and their suitability for routine clinical laboratory use is evaluated. The acidometric agar plate method is recommended.

MeSH Terms

Bacteriological Techniques Cephalosporins Evaluation Studies as Topic Haemophilus influenzae/enzymology Hydrogen-Ion Concentration Penicillinase/analysis Staphylococcus aureus/enzymology beta-Lactamases/analysis

Chemicals

Cephalosporins Penicillinase beta-Lactamases

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Lucas T J

References (13)

13 references, click to expand

A rapid test for beta-lactamase production by Haemophilus influenzae.
Lancet. 1977 Oct 29;2(8044):906 PMID: 72244
Acidometric agar plate method for ampicillin susceptibility testing of Haemophilus influenzae.
Antimicrob Agents Chemother. 1978 Feb;13(2):318-20 PMID: 306220
A rapid slide test for penicillinase.
Am J Clin Pathol. 1978 Mar;69(3):351-4 PMID: 345799
A rapid paper-strip method for the detection of penicillinase production by penicillin resistant strains of Staphylococcus aureus.
J Clin Pathol. 1978 Apr;31(4):388-9 PMID: 346615
Simple method for detection of penicillinase-producing Neisseria gonorrhoeae.
J Clin Microbiol. 1978 Jan;7(1):102-3 PMID: 415067
A rapid, simple agar-overlay method for the detection of pencillinase-producing Staphylococcus aureus in the clinical bacteriology laboratory.
Jpn J Microbiol. 1976 Apr;20(2):153-4 PMID: 781352
Susceptibility of Haemophilus influenza to ampicillin as determined by use of a modified, one-minute beta-lactamase test.
Antimicrob Agents Chemother. 1976 Jan;9(1):196-8 PMID: 1083200
Novel method for detection of beta-lactamases by using a chromogenic cephalosporin substrate.
Antimicrob Agents Chemother. 1972 Apr;1(4):283-8 PMID: 4208895
Rapid capillary tube method for detecting penicillin resistance in Staphylococcus aureus.
Appl Microbiol. 1972 Mar;23(3):649-50 PMID: 4482067
Iodometric assay of penicillinase.
Nature. 1954 Nov 27;174(4439):1012-3 PMID: 13214059
Evaluation of some currently used methods for determining staphylococcal penicillinase.
J Lab Clin Med. 1962 Mar;59:469-80 PMID: 14007995
Modified Gots test for penicillinase production.
Am J Clin Pathol. 1952 Aug;22(8):806-8 PMID: 14943698
EXTRACTION OF A HIGHLY POTENT PENICILLIN INACTIVATOR FROM PENICILLIN RESISTANT STAPHYLOCOCCI.
Science. 1944 Jun 2;99(2579):452-3 PMID: 17798398

Article Info

Journal

Journal of clinical pathology

Abbr.

J Clin Pathol

ISSN

0021-9746

DOI

10.1136/jcp.32.10.1061

Published

1979-10-00

Pages

1061-5

Language

English

Region

England

NLM ID

0376601

PMCID

PMC1145893

Subset

External Links

PubMed source DOI: 10.1136/jcp.32.10.1061 PMC Full Text View journal details

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An evaluation of 12 methods for the demonstration of penicillinase.

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