Isolation of the rec mutants in Staphylococcus aureus.

Journal of bacteriology ·Vol. 112 ·No. 3 ·1972-12-00 ·Pages 1169-76

Inoue M, Oshima H, Okubo T, Mitsuhashi S

Abstract

A histidine auxotroph (his-) of Staphylococcus aureus MS3937 and mutants sensitive to ultraviolet (UV) irradiation were obtained. The UV-sensitive mutants were found also to be sensitive to N-methyl-N'-nitro-N-nitrosoguanidine and mitomycin C, and their sensitivity was accounted for by a defect in deoxyribonucleic acid repair. Transduction of either chromosomal or plasmid markers to UV-sensitive mutants showed that these staphylococcus mutants are of the recA (reckless) type mutants as reported in Escherichia coli and Salmonella typhimurium; therefore the UV-sensitive mutants (uvr-) were renamed recombination-deficient mutants (rec-). The biochemical and genetic properties of these mutants are described, and their usefulness for studies of staphylococcal plasmids is discussed.

MeSH Terms

Carbon Isotopes Cell Survival Culture Media DNA Repair DNA, Bacterial/biosynthesis Drug Resistance, Microbial Extrachromosomal Inheritance Histidine/metabolism Mitomycins/pharmacology Mutagens Mutation Nitrosoguanidines/pharmacology Radiation Effects Recombination, Genetic Staphylococcus/drug effects,isolation & purification,metabolism,radiation effects Staphylococcus Phages/radiation effects Thymidine/metabolism Transduction, Genetic Ultraviolet Rays

Chemicals

Carbon Isotopes Culture Media DNA, Bacterial Mitomycins Mutagens Nitrosoguanidines Histidine Thymidine

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Inoue M

Oshima H

Okubo T

Mitsuhashi S

References (23)

23 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.112.3.1169-1176.1972

Published

1972-12-00

Pages

1169-76

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC251545

Subset

External Links

PubMed source DOI: 10.1128/jb.112.3.1169-1176.1972 PMC Full Text View journal details

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Isolation of the rec mutants in Staphylococcus aureus.

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