Stimulation of RNA transcription from pea and corn DNA by protein retained on sepharose coupled to 2,4-dichlorophenoxyacetic acid.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 8 ·1971-08-00 ·Pages 1824-7

Venis MA

Abstract

When crude extracts from pea or corn shoots are passed through a column of agarose coupled to a derivative of the synthetic plant hormone 2,4-dichlorophenoxyacetic acid, certain protein fractions are retained, whereas unmodified agarose adsorbs no protein. Appropriate elution yields a protein factor that stimulates DNA-dependent RNA synthesis (supported by Escherichia coli polymerase) by 40-200%. Preliminary evidence suggests that the factor may be influencing initiation of RNA chains.

MeSH Terms

2,4-Dichlorophenoxyacetic Acid Acetates Adenosine Triphosphatases/analysis Adenosine Triphosphate/metabolism Carbon Isotopes Chromatography DNA/isolation & purification,metabolism Electrophoresis Escherichia coli/enzymology Genetic Code Phenols Phosphorus Isotopes Plant Proteins/analysis,pharmacology Plants, Edible/analysis,metabolism Potassium/pharmacology Protein Binding RNA/biosynthesis RNA Nucleotidyltransferases/pharmacology Ribonucleases/analysis Rifampin/pharmacology Zea mays/analysis,metabolism

Chemicals

Acetates Carbon Isotopes Phenols Phosphorus Isotopes Plant Proteins 2,4-Dichlorophenoxyacetic Acid RNA Adenosine Triphosphate DNA RNA Nucleotidyltransferases Ribonucleases Adenosine Triphosphatases Potassium Rifampin

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Venis M A

References (13)

13 references, click to expand

The twisted circular form of polyoma viral DNA.
Proc Natl Acad Sci U S A. 1965 May;53(5):1104-11 PMID: 4287964
On the similarity of plant and animal histones.
Biochemistry. 1966 Aug;5(8):2563-70 PMID: 5968564
A receptor molecule for estrogens: studies using a cell-free system.
Proc Natl Acad Sci U S A. 1967 Jun;57(6):1740-3 PMID: 5232110
Mode of action of rafamycin on the RNA polymerase reaction.
Biochim Biophys Acta. 1968 Mar 18;157(1):218-9 PMID: 4868249
Microbial synthesis and degradation of indole-3-acetic acid. 3. The isolation and characterization of indole-3-acetyl-epsilon-L-lysine.
Biochemistry. 1968 Feb;7(2):601-5 PMID: 5644130
Enhancement of RNA synthesis in isolated pea nuclei by gibberellic acid.
Proc Natl Acad Sci U S A. 1968 Jan;59(1):269-76 PMID: 5242128
Selective enzyme purification by affinity chromatography.
Proc Natl Acad Sci U S A. 1968 Oct;61(2):636-43 PMID: 4971842
A protein intermediary in the interaction of a hormone with the genome.
Proc Natl Acad Sci U S A. 1969 Jul;63(3):897-903 PMID: 5259771
A new method for the large scale purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase.
J Biol Chem. 1969 Nov 25;244(22):6160-7 PMID: 4900510
A factor mediating interaction of kinins with the genetic material.
Biochim Biophys Acta. 1970 Feb 18;199(2):511-8 PMID: 4907338
Stimulation of chromatin-bound RNA polymerase activity by a soluble factor.
Biochim Biophys Acta. 1970 Dec 14;224(2):667-70 PMID: 5498106
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
EVIDENCE FOR A RING STRUCTURE OF POLYOMA VIRUS DNA.
Proc Natl Acad Sci U S A. 1963 Aug;50:236-43 PMID: 14060639

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.68.8.1824

Published

1971-08-00

Pages

1824-7

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC389301

Subset

External Links

PubMed source DOI: 10.1073/pnas.68.8.1824 PMC Full Text View journal details

Analysis Services

Stimulation of RNA transcription from pea and corn DNA by protein retained on sepharose coupled to 2,4-dichlorophenoxyacetic acid.

Contact

WeChat Official Account