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PMID: 4255373 Published · ppublish English Journal Article

Adenosine triphosphate--dependent calcium uptake by rat submaxillary gland microsomes.

The Journal of general physiology ·Vol. 58 ·No. 3 ·1971-09-00 ·Pages 340-50

Alonso GL, Bazerque PM, Arrigó DM, Tumilasci OR

Abstract

Microsomes from rat submaxillary glands are able to take up calcium from the suspension media. Calcium uptake is greatly increased by the presence of ATP. This effect of ATP is not detected at 0 degrees C. ADP cannot replace ATP to potentiate calcium uptake. ATP-dependent calcium uptake is not observed in the absence of magnesium. ATP-dependent calcium uptake is enhanced by oxalate and, to a lesser degree, by inorganic phosphate. Total calcium per milligram of microsomal protein observed when tests were performed without oxalate closely parallels the amounts for skeletal and cardiac muscles reported by several authors. Calcium uptake in salivary gland microsomes is slower than in muscle microsomes. Speculations are considered about the role of ATP-dependent calcium uptake. It is suggested that a decrease in intracellular free calcium levels returns these cells to the resting state after secretion.

MeSH Terms
Adenosine Diphosphate/pharmacology Adenosine Triphosphatases/metabolism Adenosine Triphosphate/pharmacology Animals Calcium/metabolism Calcium Isotopes Drug Synergism In Vitro Techniques Magnesium/pharmacology Microscopy, Electron Microsomes/drug effects,metabolism Muscles/cytology Oxalates/pharmacology Phosphates/pharmacology Potassium/pharmacology Rats Salivary Glands/cytology Sodium/pharmacology Stimulation, Chemical Submandibular Gland/cytology,enzymology Time Factors
Chemicals
Calcium Isotopes Oxalates Phosphates Adenosine Diphosphate Adenosine Triphosphate Sodium Adenosine Triphosphatases Magnesium Potassium Calcium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Alonso G L
Bazerque P M
Arrigó D M
Tumilasci O R
References (18)
18 references, click to expand
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Article Info
Journal
The Journal of general physiology
Abbr.
J Gen Physiol
ISSN
0022-1295
DOI
10.1085/jgp.58.3.340
Published
1971-09-00
Pages
340-50
Language
English
Region
United States
NLM ID
2985110R
PMCID
PMC2226031
Subset
IM
External Links
PubMed source DOI: 10.1085/jgp.58.3.340 PMC Full Text View journal details
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