Comparison of animal sera for suitability in coagulase testing.

Applied microbiology ·Vol. 21 ·No. 3 ·1971-03-00 ·Pages 420-5

Orth DS, Chugg LR, Anderson AW

Abstract

The sera of several animals were examined for suitability in coagulase testing. The assay for coagulase-reacting factor (CRF) activities of the whole sera indicated the following relative concentrations of CRF: human > pig > rabbit > horse > bovine, chicken, and lamb. Human, pig, and rabbit sera had adequate amounts of CRF for coagulase testing. The plasmin activities of the different sera, arranged from the strongest to the weakest, were as follows: rabbit > human > lamb > horse > bovine, chicken, and pig. Fibrinolysis was observed when rabbit, human, lamb, or horse sera were incorporated into coagulase test agars. Pig serum was superior to the other sera for use in the plate test for coagulase since it had adequate amounts of CRF and the plasminogen-plasmin system was not activated by staphylokinase or staphylococcal Müller factor. Heparinized pig plasma was more suitable than citrated pig plasma since citrate interfered with the growth of Staphylococcus aureus, and the use of heparinized plasma prevented false-positive coagulase reactions due to citrate utilization.

MeSH Terms

Agar Animals Arginine Bacteriological Techniques/standards Cattle Chickens Citrates Coagulase/analysis Esterases/blood False Positive Reactions Fibrinolysin/metabolism Heparin Horses Humans Immune Sera Peptide Hydrolases/blood Plasma Rabbits Sheep Staphylococcus/enzymology Streptokinase/blood Swine

Chemicals

Citrates Coagulase Immune Sera Agar Heparin Arginine Esterases Peptide Hydrolases Streptokinase Fibrinolysin

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Orth D S

Chugg L R

Anderson A W

References (13)

13 references, click to expand

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Article Info

Journal

Applied microbiology

Abbr.

Appl Microbiol

ISSN

0003-6919

DOI

10.1128/am.21.3.420-425.1971

Published

1971-03-00

Pages

420-5

Language

English

Region

United States

NLM ID

7605802

PMCID

PMC377196

Subset

External Links

PubMed source DOI: 10.1128/am.21.3.420-425.1971 PMC Full Text

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Comparison of animal sera for suitability in coagulase testing.

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