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PMID: 424302 Published · ppublish English Journal Article

Mammalian mitochondrial transfer RNAs: chromatographic properties, size and origin.

Nucleic acids research ·Vol. 6 ·No. 2 ·1979-02-00 ·Pages 455-69

Aujame L, Freeman KB

Abstract

Incubation of isolated rat liver mitochondria with radioactive amino acids resulted in the charging of tRNAs for arginine, asparagine, leucine, lysine, methionine, proline and valine. The aminoacyl-tRNAs were shown to be distinct from their cytosolic counterparts by chromatography on RPC-5. By electrophoresis on urea polyacrylamide slab gels it was found that all these mitochondrial aminoacyl-tRNAs were about 70-76 nucleotides long. The unique mitochondrial asparaginyl- and prolyl-tRNAs, not previously identified in mammalian cells, were shown to hybridize to mtDNA. Mitochondrial leucyl-tRNA separated into 3 peaks on RPC-5 and the first species was shown to be different than a combination of the other two by molecular size and partial RNase T1 digestion patterns. Each was coded by a separate gene on mtDNA as shown by partial additivity of hybridization. Separate genes for mitochondrial tRNAMetm and tRNAMetf, separated by RPC-5 chromatography, were also demonstrated. These results bring to 21 the number of individual tRNAs coded by mammalian mtDNA.

MeSH Terms
Animals Cytosol/analysis Liver/analysis Mitochondria, Liver/metabolism Molecular Weight Nucleic Acid Hybridization Protein Biosynthesis RNA, Transfer/isolation & purification,metabolism Rats
Chemicals
RNA, Transfer
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Aujame L
Freeman K B
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37 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/6.2.455
Published
1979-02-00
Pages
455-69
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327707
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/6.2.455 PMC Full Text View journal details
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