Analysis of the attachment of replicating DNA to a nuclear matrix in mammalian interphase nuclei.

Nucleic acids research ·Vol. 6 ·No. 1 ·1979-01-00 ·Pages 219-30

Dijkwel PA, Mullenders LH, Wanka F

Abstract

The attachment of replicating DNA to a rapidly sedimenting nuclear structure was investigated by digestion with various nucleases. When DNA was gradually removed by DNase I, pulse label incorporated during either 1 min or during 1 hour in the presence of arabinosylcytosine, remained preferentially attached to the nuclear structure. Single strand specific digestion by nuclease S1 or staphylococcal nuclease at low concentrations caused a release of about 30% of the pulse label, without significantly affecting the attachment of randomly labelled DNA. The released material had a low sedimentation coefficient and contained most of the Okasaki fragments. The remaining pulse label was less accessible to further digestion by double strand specific nuclease activity than the bulk DNA. The results suggest that an attachment of the replication fork to the nuclear structure occurs at sites behind but close to the branch point.

MeSH Terms

Animals Cattle Cell Cycle Cell Nucleus/metabolism Cells, Cultured DNA/biosynthesis,isolation & purification DNA Replication Deoxyribonucleases Liver/metabolism Molecular Weight

Chemicals

DNA Deoxyribonucleases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Dijkwel P A

Mullenders L H

Wanka F

References (15)

15 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/6.1.219

Published

1979-01-00

Pages

219-30

Language

English

Region

England

NLM ID

0411011

PMCID

PMC327684

Subset

External Links

PubMed source DOI: 10.1093/nar/6.1.219 PMC Full Text View journal details

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Analysis of the attachment of replicating DNA to a nuclear matrix in mammalian interphase nuclei.

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