Dodecyl sulphate/polyacrylamide-gel electrophoresis at low pH values and low temperatures.

The Biochemical journal ·Vol. 181 ·No. 3 ·1979-09-01 ·Pages 759-61

Lichtner R, Wolf HU

Abstract

A simple method is described for dodecyl sulphate/polyacrylamide-gel electrophoresis of pH- and temperature-labile biological intermediates. The method is based on a catalyst system that works at temperatures of 2--4 degrees C and pH values of 2--4 and an appropriate buffer system containing Li+ or Tris [CH2OH--C(CH2OH)2--NH3+] instead of Na+. This system does not lead to the precipitation of 1% dodecyl sulphate.

MeSH Terms

Electrophoresis, Polyacrylamide Gel/methods Hydrogen-Ion Concentration Lithium Sodium Dodecyl Sulfate Temperature Tromethamine

Chemicals

Tromethamine Sodium Dodecyl Sulfate Lithium

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Lichtner R

Wolf H U

References (15)

15 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1810759

Published

1979-09-01

Pages

759-61

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1161216

Subset

External Links

PubMed source DOI: 10.1042/bj1810759 PMC Full Text View journal details

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Dodecyl sulphate/polyacrylamide-gel electrophoresis at low pH values and low temperatures.

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