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PMID: 4223043 Published · ppublish English Journal Article

The uncoupling of respiratory-chain phosphorylation by 4,5,6,7-tetrachloro-2-trifluoromethylbenzimidazole.

The Biochemical journal ·Vol. 98 ·No. 1 ·1966-01-00 ·Pages 284-9

Beechey RB

Abstract

1. 30mmum-4,5,6,7-Tetrachloro-2-trifluoromethylbenzimidazole (TTFB) uncouples respiratory-chain phosphorylation. The respiration rate of uncoupled mitochondria is significantly greater than the state 3 rate. Neither added orthophosphate nor ATP is required to sustain this rate. The oligomycin- and octylguanidine-induced inhibitions of respiration are relieved by uncoupling concentrations of TTFB. 2. Uncoupling concentrations of TTFB elicit a high adenosinetriphosphatase activity and inhibit the ATP-dependent succinate-linked reduction of NAD catalysed by submitochondrial particles from ox-heart mitochondria. 3. The action of TTFB is similar to that of 2,4-dinitrophenol. Evidence is presented which shows that it is the anionic forms of benzimidazoles and imidazoles, having the negative charge localized on the ring nitrogen atom, which are the effective uncoupling agents.

MeSH Terms
Adenosine Triphosphatases/metabolism Animals Antimetabolites/pharmacology Benzimidazoles/pharmacology Cattle Chromatography, Thin Layer Guanidines/pharmacology In Vitro Techniques Manometry Mitochondria/enzymology,metabolism Myocardium/cytology Oligomycins/pharmacology Oxidative Phosphorylation/drug effects Rats
Chemicals
Antimetabolites Benzimidazoles Guanidines Oligomycins Adenosine Triphosphatases
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Beechey R B
References (18)
18 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj0980284
Published
1966-01-00
Pages
284-9
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1264827
Subset
IM
External Links
PubMed source DOI: 10.1042/bj0980284 PMC Full Text View journal details
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