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PMID: 4215759 Published · ppublish English Journal Article

Intracellular and extracellular degranulation of human polymorphonuclear azurophil and specific granules induced by immune complexes.

Infection and immunity ·Vol. 10 ·No. 6 ·1974-12-00 ·Pages 1241-9

Leffell MS, Spitznagel JK

Abstract

The nature of the phagocytic stimulus determines the degree of degranulation. Specifically, immune complexes induced more degranulation than did antigen or antibody alone. The release of constituents from the two granule classes represented independent phenomena. Relatively more of the specific granule proteins were released into the medium during phagocytosis, whereas more of the azurophil proteins were associated with the phagosomes.

MeSH Terms
Alkaline Phosphatase/blood Animals Antigen-Antibody Complex Cell Separation Centrifugation, Density Gradient Chromatography, Affinity Complement Fixation Tests Cytoplasmic Granules/enzymology,immunology Glucuronidase/blood Humans Immune Sera Immunodiffusion Immunoglobulin G Iodine Radioisotopes L-Lactate Dehydrogenase/blood Latex Lysosomes Microspheres Neutrophils/immunology Peptide Hydrolases/analysis Peroxidases/blood Phagocytosis Rabbits/immunology Serum Albumin, Bovine
Chemicals
Antigen-Antibody Complex Immune Sera Immunoglobulin G Iodine Radioisotopes Latex Serum Albumin, Bovine L-Lactate Dehydrogenase Peroxidases Alkaline Phosphatase Glucuronidase Peptide Hydrolases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Leffell M S
Spitznagel J K
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40 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.10.6.1241-1249.1974
Published
1974-12-00
Pages
1241-9
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC423094
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.10.6.1241-1249.1974 PMC Full Text View journal details
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